PCR sample preparation holder and method
A simplified Polymerase Chain Reaction (PCR) sample preparation apparatus and method of using a PCR sample preparation for both liquid and dry samples. A portable apparatus comprising a handle removably attached to a wand assembly. The handle includes a cover and a swab with an absorbent material. The wand assembly includes a buffer container for a buffer that is ruptured by a spike when the holder is inserted into the wand assembly. The apparatus may include a waste container removably attached to a tube comprising a plunger, a safety clip to prevent the plunger from depressing, and a port for introduction of a liquid sample. A filter on the waste container collects a specimen of the liquid sample for preparation as described above.
1. An apparatus for preparing samples for Polymerase Chain Reaction analysis comprising:
a handle;
a swab, including a chamber, attached to an end of the handle, wherein the swab includes a stabilized reagent in the chamber and a sample tube extending from an end of the chamber; and
a wand assembly including a buffer container at an end of a shaft and a stand at another end of the shaft;
wherein the handle is configured to be attached to the wand assembly, and
wherein the wand assembly includes an extendable grip.
2. The apparatus of claim 1 , wherein the buffer container includes a film covering one end of the container.
3. The apparatus of claim 1 , further comprising a spike positioned in the wand assembly and configured to rupture the buffer container to allow a buffer to exit the buffer container and flow into the chamber.
4. The apparatus of claim 1 , wherein the chamber includes vents.
5. The apparatus of claim 1 , wherein the handle removably attaches to the wand assembly by the swab inserting into the shaft of the wand assembly.
6. The apparatus of claim 5 , wherein a cover of the handle is configured to detach from the swab when the swab is attached to the wand assembly.
7. The apparatus of claim 1 , wherein the stand is configured to attach to a slot in a hand-held detector for biological materials.
8. The apparatus of claim 1 , wherein the handle and wand assembly are configured to be portable.
9. The apparatus of claim 1 , further comprising an absorbent material attached at an end of the swab.
10. The apparatus of claim 9 , wherein the absorbent material is held in place by a retaining ring.
11. The apparatus of claim 9 , wherein a mesh covers the absorbent material.
12. The apparatus of claim 9 , further comprising a mesh underneath the absorbent material.
13. The apparatus of claim 9 , wherein the absorbent material comprises paper.
14. A biological sampling method for dry and/or processed samples, comprising the following steps:
providing a handle containing an absorbent material and stabilized reagent and wiping the absorbent material over a test surface; and
providing a wand assembly and inserting the handle into the wand assembly, thereby rupturing a buffer container to enable a buffer to be released through the absorbent material and into a chamber for mixing with the reagent and filling a sample tube;
sealing the chamber by fully inserting a swab of the handle into the wand assembly;
extending a grip on the wand;
removing a cover on the swab, and moving the wand by executing two whip actions wherein one end of the wand is moved over an arcuate distance that is greater than another end of the wand;
retracting the grip on the wand; and
inserting the wand into a hand-held detector for biological materials.
15. The method of claim 14 , wherein the buffer container is ruptured by rupturing a film covering one end of the container.
16. The method of claim 14 , wherein the absorbent material comprises paper.
17. The method of claim 14 , wherein the absorbent material is covered by a mesh.
18. The method of claim 14 , further comprising a mesh under the absorbent material.
19. An apparatus for preparing samples for Polymerase Chain Reaction analysis comprising:
a handle;
a swab, including a chamber, attached to the handle, wherein the swab includes a reagent in the chamber and a sample tube in fluid communication with the chamber; and
a wand assembly including an extendable grip and a buffer container containing a buffer, the apparatus being adapted to direct the buffer from the buffer container and into the chamber;
wherein the handle is configured to be attached to the wand assembly.
20. The apparatus of claim 19 , wherein the buffer container includes a film covering one end of the container.
21. The apparatus of claim 19 , further comprising a spike positioned in the wand assembly and configured to rupture the buffer container to allow a buffer to exit the buffer container and flow into the chamber.
22. The apparatus of claim 19 , wherein the chamber includes vents.
23. The apparatus of claim 19 , wherein the handle removably attaches to the wand assembly by the swab inserting into the shaft of the wand assembly.
24. The apparatus of claim 23 , wherein a cover of the handle is configured to detach from the swab when the swab is attached to the wand assembly.
25. The apparatus of claim 19 , wherein the handle and wand assembly are configured to be portable.
26. The apparatus of claim 19 , further comprising an absorbent material attached at an end of the swab.
27. The apparatus of claim 26 , wherein the absorbent material is held in place by a retaining ring.
28. The apparatus of claim 26 , wherein a mesh covers the absorbent material.
29. The apparatus of claim 26 , further comprising a mesh underneath the absorbent material.