Crystallization of Glucocorticoid Receptor
Provided are crystals relating to human Glucocorticoid Receptor and its various uses.
1. A composition comprising a protein in crystalline form wherein the protein consists of SEQ ID NO:3, wherein said proteins forms a complex with a steroid ligand, and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 space group and unit cell dimensions, +/−5%, of a=202.02 Å, b=110.31 Å and c=116.62 Å, α=β=γ=90.
2. A composition according to claim 1 wherein the crystal diffracts X-rays for a determination of structure coordinates to a resolution of a value equal to or less than 3.0 Angstroms.
3. A method for forming a crystal of a protein comprising:
forming a crystallization volume comprising: a precipitant solution and a protein that consists of SEQ ID NO:3, wherein said proteins forms a complex with a steroid ligand, and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 space group and unit cell dimensions, +/−5%, of a=202.02 Å, b=110.31 Åand c=116.62 Å, α=β=γ=90, and
storing the crystallization volume under conditions suitable for crystal formation of the protein.
4. A method according to claim 3 wherein the crystal diffracts X-rays for a determination of structure coordinates to a resolution of a value equal to or less than 3.0 Angstroms.
5. A method according to claim 3 , the method further comprising diffracting the protein crystal to produce a diffraction pattern.
6. An isolated soluble protein consisting of residues 528-777 of SEQ ID NO:1.
7. The protein according to claim 6 wherein the protein is expressed from a nucleic acid molecule that consists of SEQ ID NO:2.
8. A soluble protein consisting of SEQ ID NO:3.
9. An isolated non-crystalline protein consisting of amino acids 528-777 of SEQ ID NO:1.