Crystallization of MvaS (HMG-CoA Synthase)
Provided are crystals relating to MvaS (HMG-CoA Synthase) and its various uses.
1. A composition comprising a protein in crystalline form wherein the protein consists of SEQ ID NO:1 and wherein the protein crystal has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=68.7 b=79.6 c=150.2, α=β=γ=90.
2. A composition according to claim 1 wherein the protein crystal diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
3. A method comprising:
forming a crystallization volume comprising a precipitant solution and a protein consisting of SEQ ID NO:1, wherein a protein crystal is formed that has a crystal lattice in a P2 1 2 1 2 1 space group and unit cell dimensions, +/−5%, of a=68.7 b=79.6 c=150.2, α=ββγ=90; and
storing the crystallization volume under conditions suitable for crystal formation of the protein.
4. A method according to claim 3 wherein a protein crystal is formed that diffracts X-rays for a determination of structure coordinates to a resolution greater than 3.0 Angstroms.
5. A method according to claim 3 , wherein a protein crystal is formed, the method further comprising diffracting the protein crystal to produce a diffraction pattern and solving the structure of the protein from the diffraction pattern.