Compositions and methods for detecting and quantifying COX-2 activity by lipoamino acid metabolism
A method of detecting an activity of a COX-2 enzyme in a subject that includes obtaining a sample of the subject; detecting an amino acid eicosanoid metabolite in the sample, wherein the presence of the amino acid eicosanoid metabolite indicates the activity of the COX-2 enzyme of the subject. Preferably the amino acid eicosanoid metabolite is a PGH 2 -Gly or HETE-Gly metabolite. The metabolite may be detected based on metabolism of a COX-2-selective substrate. Preferably, the substrate is a lipoamino acid. More preferably, the lipoamino acid is selected from NAGly, N-arachidonyl-alanine, and δ-arachidonyl aminobutuyic acid.
1. A method of detecting COX-2 enzymatic activity in a subject, comprising:
a. obtaining a sample of the subject;
b. detecting an amino acid eicosanoid metabolite in the sample, wherein the presence of the amino acid eicosanoid metabolite indicates the enzymatic activity of the COX-2 enzyme of the subject, wherein the amino acid eicosanoid metabolite is PGH2-Gly or HETE-Gly metabolite.
2. The method of claim 1 , wherein the sample is urine.
3. The method of claim 1 , wherein the sample is selected from the group consisting of blood, plasma, cerebrospinal fluid, saliva, bile, sputum, joint fluid, biopsy, and media from a cell culture.
4. The method of claim 1 , wherein the amino acid eicosaniod metabolite is detected based on oxygenation of N-arachidonylglycine.
5. The method of claim 1 , wherein the detecting step comprises generating mass spectrum.
6. A method of detecting enzymatic activity of COX-2 in a subject, comprising:
a. obtaining a sample of the subject; and
b. detecting a COX-2 specific metabolite of N-arachidonylglycine selected from the group consisting of PGH2-Gly and HETE-Gly wherein said metabolite comprises an eicosanoid, wherein the presence of the metabolite in the sample indicates the enzymatic activity of COX-2 in the subject.
7. The method of claim 6 , wherein the eicosanoid is at least one of PGH2-glycine and HETE-Gly.
8. The method of claim 6 , wherein the sample comprises urine.
9. The method of claim 6 , wherein the sample is selected from a group consisting of urine, plasma, cerebrospinal fluid, saliva, sputum, bile, joint fluid, biopsy, and conditioned media from a cell culture.
10. The method of claim 6 , wherein the detecting step comprises generating a mass spectrum of the metabolite.