Polynucleotides and polypeptides in plants
The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
1. A transgenic plant that has greater tolerance to cold than a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant; wherein the transgenic plant comprises a recombinant polynucleotide encoding a polypeptide, and wherein the polypeptide comprises a conserved Myb DNA-binding domain with at least 95% sequence identity to the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO: 438, and wherein the conserved Myb DNA-binding domain binds DNA.
2. The transgenic plant of claim 1 , wherein the transgenic plant is more tolerant to 8° C. during its germination than the control plant.
3. The transgenic plant of claim 1 , wherein the transgenic plant is more tolerant to 8° C. during its development than the control plant.
4. The transgenic plant of claim 1 , wherein the conserved domain has at least 98% sequence identity with the conserved domain of amino acid coordinates 13-116 of SEQ ID NO: 438.
5. The transgenic plant of claim 1 , wherein the polypeptide is encoded by a nucleic acid that is derived from a monocotyledonous plant.
6. The transgenic plant of claim 5 , wherein the transgenic plant is a eudicot.
7. The transgenic plant of claim 1 , wherein the polypeptide is encoded by a nucleic acid that is derived from a eudicot plant.
8. The transgenic plant of claim 7 , wherein the transgenic plant is monocotyledonous.
9. The transgenic plant of claim 1 , wherein the recombinant polynucleotide further comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide.
10. An expression vector for expression of a polypeptide that confers greater cold tolerance in a transgenic plant than the cold tolerance of a control plant wherein the expression vector comprises:
(1) a constitutive, inducible, or tissue-specific promoter; and
(2) a recombinant polynucleotide encoding the polypeptide that regulates transcription, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO: 438 and wherein the conserved Myb DNA-binding domain binds DNA; wherein the constitutive, inducible, or tissue-specific promoter regulates expression of the polypeptide.
11. The expression vector of claim 10 , wherein the conserved domain of the polypeptide has at least 98% sequence identity with the conserved domain of amino acid coordinates 13-116 of SEQ ID NO: 438.
12. The expression vector of claim 10 , wherein the recombinant polynucleotide comprises SEQ ID NO: 437.
13. A host plant cell comprising the expression vector of claim 10 .
14. A method for producing a transgenic plant having greater tolerance to cold as compared to a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant, wherein the control plant is without the recombinant polynucleotide, the method comprising:
(a) providing an expression vector encoding the polypeptide, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO: 438 and wherein the conserved Myb DNA-binding domain binds DNA; and
(b) introducing the expression vector into a first target plant to produce the transgenic plant, or introducing the expression vector into a cell of a second target plant, and growing the cell into the transgenic plant.
15. The method of claim 14 , wherein the conserved domain of the polypeptide has at least 98% sequence identity with the conserved domain of amino acid coordinates 13-116 of SEQ ID NO: 438.
16. The method of claim 14 , wherein the expression vector comprises SEQ ID NO: 437.
17. The method of claim 14 , wherein:
the transgenic plant is selected by comparing cold tolerance of the transgenic plant with cold tolerance of the control plant.
18. The method of claim 14 , wherein the transgenic plant is more tolerant to 8° C. during its germination than the control plant.
19. The method of claim 14 , wherein the transgenic plant is more tolerant to 8° C. during its development than the control plant.
20. The method of claim 14 , wherein the expression vector further comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide.
21. A transgenic seed comprising an expression vector for expression of a polypeptide that confers greater cold tolerance in a transgenic plant relative to a control plant, wherein the expression vector comprises:
(1) a constitutive, inducible, or tissue-specific promoter; and
(2) a recombinant polynucleotide encoding the polypeptide that regulates transcription, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO: 438 and wherein the conserved Myb DNA-binding domain binds DNA; and wherein the constitutive, inducible, or tissue-specific promoter regulates expression of the polypeptide.
22. The transgenic seed of claim 21 , wherein the conserved domain of the polypeptide has at least 98% sequence identity with a conserved domain of amino acid coordinates 13-116 of SEQ ID NO: 438.
23. A transgenic plant that has greater tolerance to cold as compared to a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant, wherein the transgenic plant comprises:
a recombinant polynucleotide encoding a polypeptide that regulates transcription; and the polypeptide comprises the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO:438 and wherein the conserved Myb DNA-binding domain binds DNA.
24. The transgenic plant of claim 23 , wherein the transgenic plant is more tolerant to 8° C. during its germination than the control plant.
25. The transgenic plant of claim 23 , wherein the transgenic plant is more tolerant to 8° C. during its development than the control plant.
26. A method for producing a transgenic plant having greater tolerance to cold as compared to a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant, wherein the control plant is without the recombinant polynucleotide, the method comprising:
(a) providing an expression vector encoding a polypeptide comprising the Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO: 438 and wherein the conserved Myb DNA-binding domain binds DNA; and
(b) introducing the expression vector into a plant or a cell of a plant, thereby producing the transgenic plant.
27. The method of claim 26 , wherein the expression vector further comprises a constitutive, inducible, or tissue-specific promoter that regulates expression of the polypeptide.
28. The method of claim 26 , wherein the transgenic plant is more tolerant to 8° C. during its germination than the control plant.
29. The method of claim 26 , wherein the transgenic plant is more tolerant to 8° C. during its development than the control plant.