Process for producing phosphorylase
It is intended to provide a process for conveniently producing phosphorylase at a high purity using a phosphorylase-producing microorganism. Namely, a process for producing phosphorylase characterized by culturing a phosphorylase-producing microorganism in a medium containing phosphoric acid or its salt at a concentration of 50 mM or above and collecting the phosphorylase thus produced in the medium.
1. A process for producing phosphorylase, which comprises:
culturing a phosphorylase-producing microorganism in a medium comprising 50 mM or higher concentration of phosphoric acids or the salts thereof, and
collecting the phosphorylase produced in the medium,
wherein the phosphorylase-producing microorganisms are bacteria belonging to genus Leuconostoc or genus Corynebacterium , and
wherein the phosphorylase is selected from at least one of the group consisting of:
sucrose phosphorylase and maltodextrin phosphorylase.
2. The process according to claim 1 , wherein said phosphoric acid is selected from the one or more of the group consisting of: phosphoric acid, metaphosphoric acid, trypoliphosphoric acid, polyphosphoric acid, diphosphoric acid, polymetaphosphoric acid and the salts thereof.
3. The process according to claim 1 , wherein said phosphoric acid salt is selected from the one or more of the group consisting of: monobasic potassium phosphate, dibasic potassium phosphate, monobasic sodium phosphate and dibasic sodium phosphate.
4. The process according to claim 1 , wherein said phosphoric acid is present in the medium at a concentration of from 400 mM to 1.2 M for Leuconostoc or from 100 mM to 600 mM for Corynebacterium.
5. The process according to claim 1 , wherein the collecting step is performed by one or more of the following procedures selected from the group consisting of: ultrafiltration, salting-out, ion exchange, hydrophobic chromatography, gel filtration and drying.
6. A process for producing sucrose phosphorylase from Leuconostoc , which comprises:
culturing Leuconostoc in a medium comprising from 400 mM to 1.2 M concentration of a phosphoric acid or a salt thereof, and
collecting sucrose phosphorylase produced in the medium.
7. A process for producing maltodextrin phosphorylase from Corynebacterium , which comprises:
culturing Corynebacterium in a medium comprising from 100 mM to 600 mM concentration of a phosphoric acid or a salt thereof, and
collecting maltodextrin phosphorylase produced in the medium.
8. The process according to claim 6 , wherein said Leuconostoc is selected from the group consisting of: L. mesenteroides JCM9693, L. mesenteroides KSM-SP1, L. mesenteroides KSM-SP78, L. carnosum JCM9695, L. argentinum JCM 1 1052 and L. pseudomesenteroides JCM11045.
9. The process according to claim 7 , wherein said Corynebacterium is selected from the group consisting of: c. vitaeruminis JCM1323, c. callunae IFO15359, c. glutainicum JCM1321 and c. glutamicum KSM-MP669.