Intron associated with myotonic dystrophy type 2 and methods of use
The present invention provides methods for identifying individuals not at risk for developing myotonic dystrophy type 2 (DM2), and individuals that have or at risk for developing DM2. The present invention also provides isolated polynucleotides that include a repeat tract within intron 1 of the zinc finger protein 9.
1. An isolated polynucleotide comprising about nucleotides 17501-17701 of SEQ ID NO:1 and an at risk repeat tract, and the full complements thereof.
2. An isolated polynucleotide comprising about nucleotides 17858-18062 of SEQ ID NO:1 and an at risk repeat tract, and the full complements thereof.
3. The isolated polynucleotide of claim 1 wherein the at risk repeat tract comprises (TG) x (TCTG) y (CCTG) z ,
wherein x is an integer from 14 to 25, y is an integer from 3 to 10, and z is an integer from 75 to 11,000.
4. The isolated polynucleotide of claim 1 wherein the at risk repeat tract comprises from 75 to 11,000 CCTG repeats.
5. The isolated polynucleotide of claim 4 wherein the at risk repeat tract comprises at least 75 CCTG repeats uninterrupted by other nucleotides.
6. The isolated polynucleotide of claim 2 wherein the at risk repeat tract comprises (TG) x (TCTG) y (CCTG) z ,
wherein x is an integer from 14 to 25, y is an integer from 3 to 10, and z is an integer from 75 to 11,000.
7. The isolated polynucleotide of claim 2 wherein the at risk repeat tract comprises from 75 to 11,000 CCTG repeats.
8. The isolated polynucleotide of claim 7 wherein the at risk repeat tract comprises at least 75 CCTG repeats uninterrupted by other nucleotides.