IP Library › Granted Patent US 7,494,807
Granted Patent B2
US 7,494,807 · App. 10/661,455 · Granted Feb 24, 2009

Mammalian megakaryocyte progenitor cell

Assignee: The Board of Trustees of the Leland Stanford Junior University
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Quick Facts
Patent No.
US 7,494,807
App. No.
10/661,455
Granted
Feb 24, 2009
Kind
B2
Abstract

A substantially enriched mammalian hematopoietic cell population is provided, which is characterized as a progenitor cell committed to the megakaryocyte lineage. Methods are provided for the isolation and culture of these cells. The cell enrichment methods employ reagents that specifically recognize CD9 and CD41, in conjunction with other markers expressed on lineage committed progenitor cells. These cells give rise exclusively to megakaryocytes and platelets, as evidenced by their growth and differentiation in vitro and in vivo.

Claims (4)

1. A method of enrichment for a composition of mammalian megakaryocyte progenitor cells, the method comprising:

combining reagents that specifically recognize CD41, CD9, CD34, CD2; CD3; CD4; CD7; CD8; CD10; CD11b; CD14; CD19; CD20; CD56; and glycophorin A with a sample; said sample selected from the group consisting of bone marrow and mobilized peripheral blood; and

selecting for those cells that express CD41, CD, and CD34 and do not express CD2; CD3; CD4; CD7; CD8; CD10; CD11b; CD14; CD19; CD20; CD56; and glycophorin A,

thereby providing a composition of mammalian megakaryocyte progenitor cells.

Assignments (3)
CONFIRMATORY LICENSE Recorded Jul 28, 2010
From: STANFORD UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 024753/0401 →
CONFIRMATORY LICENSE Recorded Nov 4, 2008
From: STANFORD UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 021779/0938 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 31, 2005
From: NAKORN, THANYAPHONG NA; MIYAMOTO, TOSHIHIRO; WEISSMAN, IRVING L.
To: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Reel/Frame 016080/0150 →
Continuity (2)
Provisional Application 6041049000 · Sep 13, 2002
Related Publication 20050176142A1 · Aug 11, 2005