Root-specific phosphate transporter promoters
The current invention provides plant promoter sequences. Compositions comprising the promoter sequence are described, as are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the promoters by genetic transformation, as well as by plant breeding methods.
1. An isolated nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleic acid sequence of SEQ ID NO: 1, or a fragment thereof having promoter activity;
(b) a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence of SEQ ID NO: 1, wherein the nucleic acid sequence has promoter activity; and
(c) a nucleic acid sequence fully complementary to any of (a)-(b);
wherein the isolated nucleic acid sequence is operably linked to a heterologous coding sequence.
2. The isolated nucleic acid sequence of claim 1 , further defined as operably linked to an enhancer.
3. The isolated nucleic acid sequence of claim 1 , further defined as comprising a nucleic acid sequence of SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22 or SEQ ID NO:23.
4. A transformation construct comprising the isolated nucleic acid sequence of claim 1 operably linked to a heterologous coding sequence.
5. The transformation construct of claim 4 , wherein the coding sequence is operably linked to a terminator.
6. The transformation construct of claim 4 , further comprising an enhancer.
7. The transformation construct of claim 4 , further comprising a selectable marker.
8. The transformation construct of claim 4 , further comprising at least a second promoter.
9. The transformation construct of claim 8 , further comprising at least a second heterologous coding sequence operably linked to said second promoter.
10. The transformation construct of claim 4 , further comprising a screenable marker.
11. A plant transformed with a selected DNA comprising the promoter sequence of claim 1 .
12. The plant of claim 11 , further defined as a dicotyledonous plant.
13. The plant of claim 11 , further defined as a monocotyledonous plant.
14. A cell of the plant of claim 11 .
15. A seed of the plant of claim 11 , wherein said seed comprises said selected DNA.
16. A progeny plant of any generation of the plant of claim 11 , wherein said progeny plant comprises said selected DNA.
17. A method of expressing a polypeptide in a plant cell comprising the steps of:
(a) obtaining a construct comprising the promoter of claim 1 operably linked to a heterologous coding sequence encoding a polypeptide; and
(b) transforming a recipient plant cell with the construct, wherein said recipient plant cell expresses said polypeptide.
18. The method of claim 17 , wherein the plant cell is further defined as a dicotyledonous plant cell.
19. The method of claim 17 , wherein the plant cell is further defined as a monocotyledonous plant cell.
20. A method of producing a plant transformed with a selected DNA comprising the isolated nucleic acid sequence of claim 1 operably linked to a heterologous coding sequence, comprising:
(a) obtaining a first plant comprising said selected DNA;
(b) crossing said first plant to a second plant lacking said selected DNA; and
(c) obtaining at least a first progeny plant resulting from said crossing, wherein said progeny plant has inherited said selected DNA.
21. The method of claim 20 , wherein the plant is further defined as a dicotyledonous plant.
22. The method of claim 20 , wherein the progeny plant is a monocotyledonous plant.