Promoter for expression of a gene and response to infection or wounding
Promoters which increase the pathogen-inducibility of genes in plants, preferably in sugar beets, rape, potatoes, corn, soybeans, wheat, rice, rye, barley, cotton and sunflower. The promoters are characterized in that they lead to the rapid local expression of the gene respectively under the control thereof in different organs and tissues of transgenic plants such as leaves, shoots and/or roots, after having been infected by a pathogen or wounded. This attribute makes these sequences ideal for use as a regulation element for the controlled expression of antifungal compounds and thus for developing fungus-resistant plants.
1. An isolated nucleic acid promoter comprising a nucleotide sequence for elevating the defensive reaction of a plant against pathogen infection, wherein the nucleic acid promoter comprises SEQ ID NO:1.
2. The nucleic acid promoter according to claim 1 , further comprising the nucleotide sequence consisting of SEQ ID NO:24 extending beyond the 3′ end of SEQ ID NO:1.
3. A gene construct, which comprises a nucleotide sequence encoding a protein operably linked to a promoter, wherein the protein is a pathogen defense protein and the promoter comprises the nucleic acid promoter according to claim 1 .
4. The gene construct according to claim 1 , wherein the gene construct enhances local gene expression, which results in direct or indirect antifungal effect.
5. A method for producing a pathogen tolerant plant, said method comprising transforming the genome of a plant with the gene construct acid according to claim 3 .
6. A transgenic plant cell comprising the nucleic acid promoter according to claim 1 .
7. A transgenic plant, the plant comprising the plant cell according to claim 6 .
8. The transgenic plant according to claim 7 , wherein the plant is a dicotyledonous plant, and wherein said plant is from a species selected from the group consisting of Fabaceae, Ranunculaceae, Brassicaceae, Chenopodiaceen, Solanaceen, Lycopersicon, Daucus, Gossypium , and Helianthus.
9. The transgenic plant according to claim 7 , wherein said plant is a monocotyledonous plant, and wherein said plant is from a species selected from the group consisting of Poaceae, Zea mays, Triticum, Avena, Secale , and Oryza.
10. A seed of a transgenic plant comprising a plant cell, wherein said cell comprises a nucleic acid promoter comprising SEQ ID NO:1.