Plants with delayed flowering
The invention provides plants with delayed flowering and methods of making such plants. Also provided are constructs comprising nucleic acids for making these plants. The invention is significant in that plants with delayed flowering may exhibit increased nutritional value and cold tolerance. Delayed flowering will also find use in plant breeding methods.
1. An isolated nucleic acid sequence operably linked to a heterologous promoter, wherein the isolated nucleic acid sequence is selected from the group consisting of:
(a) a nucleic acid sequence encoding the polypeptide of SEQ ID NO:2;
(b) a nucleic acid sequence comprising the sequence of SEQ ID NO: 1;
(c) a nucleic acid sequence encoding a polypeptide with at least 90% sequence identity to SEQ ID NO:2, wherein ectopic expression of said nucleic acid in a plant causes said plant to have delayed flowering compared to a control plant and;
(d) the complement of the sequence of any of (a)-(c).
2. A recombinant vector comprising the isolated nucleic acid sequence of claim 1 .
3. The recombinant vector of claim 2 , further comprising at least one additional sequence chosen from the group consisting of: a regulatory sequence, a selectable marker, a leader sequence and a terminator.
4. The recombinant vector of claim 3 , wherein the additional sequence is a heterologous sequence.
5. The recombinant vector of claim 2 , wherein the promoter is a developmentally-regulated, organelle-specific, inducible, tissue-specific, constitutive, cell-specific, seed specific, or germination-specific promoter.
6. The recombinant vector of claim 2 , defined as an isolated expression cassette.
7. A transgenic plant transformed with a selected DNA comprising the nucleic acid sequence of claim 1 .
8. The transgenic plant of claim 7 , further defined as an R 0 transgenic plant.
9. The transgenic plant of claim 7 , further defined as a progeny plant of any generation of an R 0 transgenic plant, wherein said transgenic plant has inherited said selected DNA from said R 0 transgenic plant.
10. A seed of the transgenic plant of claim 7 , wherein said seed comprises said selected DNA.
11. A host cell transformed with a selected DNA comprising the nucleic acid sequence of claim 1 , wherein the host cell is a bacterial or plant cell.
12. The host cell of claim 11 , wherein said host cell expresses a protein encoded by said selected DNA.
13. The host cell of claim 11 , wherein the cell has inherited said selected DNA from a progenitor of the cell.
14. The host cell of claim 11 , wherein the cell has been transformed directly with said selected DNA.
15. The host cell of claim 11 , wherein said host cell is a plant cell.
16. A method of delaying flowering in a plant comprising introducing into the plant the isolated nucleic acid sequence of claim 1 , wherein the nucleic acid is expressed in the plant to delay flowering relative to a plant of the same genotype that lacks the isolated nucleic acid.
17. The method of claim 16 , wherein the isolated nucleic acid sequence is from a species selected from the group consisting of: Arabidopsis thaliana, barley, cotton, grape, maize, potato, rice, sugarcane, sorghum, soybean, tomato, wheat and Medicago truncatula.
18. The method of claim 16 , wherein introducing the isolated nucleic acid comprises plant breeding.
19. The method of claim 16 , wherein introducing the isolated nucleic acid comprises genetic transformation.