Method for promoting hard tissue formation
Formulations and methods to promote biological processes to form or regenerate new hard tissues such as bones, cartilage, and/or dental tissues are disclosed. The formulation comprising two proteins is administered to enhance biological activities of a hard tissue growth and differentiation factor characterized by specific and selective upregulation and/or extension of the retention time of the intracellular enzymes and signaling molecules that play important roles to proliferate, differentiate, maturate, and/or mineralize the hard tissue forming cells.
1. A method of enhancing hard tissue formation activity, comprising:
administering to a patient an effective amount of the peptide of SEQ ID NO:8; and
further administering to the patient an effective amount of recombinant human bone morphogenic protein-2 (rhBMP-2);
wherein the ratio of the rhBMP-2 to the peptide of SEQ ID NO:8 is in a range of 1:5 or more allowing the peptide and the protein to act on hard tissue forming cells and thereby enhance hard tissue formation activity.
2. The method of claim 1 wherein the hard tissue forming cells are cells chosen from the group of: (a) cells of a lineage of osteoblast wherein the hard tissue is in the form of bone tissue; (b) cells of a lineage of ameloblast wherein the hard tissue is in the form of enamel; (c) cells of a lineage of odontoblast wherein the hard tissue is in the form of dentin; and (d) cells of a lineage of cementoblast wherein the hard tissue is in the form of cementum.
3. The method of claim 1 , wherein the peptide and the protein are administered by injection.
4. The method of claim 1 , wherein the peptide and the protein are administered at the same time.
5. The method of claim 4 , wherein the peptide and the protein are administered on an absorbable collagen sponge (ACS).