Method for producing ubiquinone-10 in plant
A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.
1. A plant which is transformed using an expression cassette which expresses decaprenyl diphosphate synthase, encoded by:
a nucleic acid encoding a polypeptide having an amino acid sequence as set forth in SEQ ID NO:6,
wherein said plant is a Gramineae plant which produces ubiquinone-9,
wherein the decaprenyl diphosphate synthase maintains a physiological activity of producing ubiquinone-10 at a level greater than the level of ubiquinone-9 produced; and
wherein an amount of ubiquinone-10 larger than that of ubiquinone-9 is accumulated in the plant.
2. A plant according to claim 1 , wherein a nucleic acid encoding the decaprenyl diphosphate synthase is operatively linked with a nucleic acid encoding a mitochondrial targeting sequence.
3. A plant according to claim 2 , wherein the nucleic acid encoding the mitochondrial targeting sequence is selected from the group consisting of a nucleic acid encoding a fragment of rice RPS10 protein (SEQ ID NO. 2), a nucleic acid encoding a fragment of RPS14 protein (SEQ ID NO. 4), a nucleic acid encoding a fragment of RPS11 protein, a nucleic acid encoding a fragment of ATPase β-subunit protein (SEQ ID NO. 10), and a nucleic acid encoding a fragment of ATPase g-subunit protein (SEQ ID NO. 12).
4. A plant according to claim 2 , wherein the mitochondrial targeting sequence is a fragment consisting of amino acids 1 to 56 of rice RPS10 protein (SEQ ID NO. 2).
5. A plant according to claim 2 , wherein the mitochondrial targeting sequence is a fragment consisting of an amino acid sequence of amino acids 1 to 48 of rice RPS14 protein (SEQ ID NO. 4).
6. A plant according to claim 1 , wherein the decaprenyl diphosphate synthase gene is operatively linked with a seed-specific promoter.
7. A plant according to claim 1 , wherein the plant is selected from the group consisting of a plant cell, a plant culture cell, a plant seed, a regenerated plant, a plant callus, plant tissue, a leaf, a stem, a root, a flower, and a seeding.
8. A seed from a plant according to any one of claims 1 , 2 , 3 , 4 , 5 , or 6 , wherein the seed comprises exogenous decaprenyl diphosphate synthase.
9. A plant which contains an expression cassette which expresses decaprenyl diphosphate synthase encoded by a nucleic acid encoding a polypeptide having an amino acid sequence set forth in SEQ ID NO:6,
wherein said plant is a Gramineae plant which produces ubiquinone-9, and
wherein the decaprenyl diphosphate synthase maintains physiological activity of producing ubiquinone-10 at a level greater than the level of ubiquinone-9 produced, wherein an amount of ubiquinone-10 larger than that of ubiquinone-9 is accumulated in the plant.
10. A seed of a plant according to claim 9 , wherein the seed comprises exogenous decaprenyl diphosphate synthase.
11. A method for producing ubiquinone-10 using a plant, comprising: preparing a plant according to claim 1 ; and culturing the plant.
12. Plant tissue which is obtained from a plant according to claim 1 and contains ubiquinone-10.
13. A plant cell which is obtained from a plant according to claim 1 and contains ubiquinone-10.
14. A plant according to claim 1 , wherein the decaprenyl diphosphate synthase is encoded by the sequence complementary to SEQ ID NO. 5.