System and method for genetically treating cardiac conduction disturbances
The present invention provides delivery systems for and methods of delivering conduction protein genetic material to cardiac cells in localized areas of the heart to improve the conductance therein. More specifically, there is provided a system and method for delivering connexin proteins or nucleic acid molecules encoding connexin proteins to a site in the heart which has been determined by mapping procedures to have a conduction disturbance. For cases where conduction is impaired, selected genetic material is delivered to cells around the disturbance area, in order to enhance overall conductivity patterns; in other cases, genetic material is selected to slow conduction in affected areas, so as to prevent, e.g., brady-tachy syndrome.
1. An implantable delivery system for delivering doses of genetic material to cardiac tissue, comprising:
recombinant nucleic acid vectors encoding a conduction protein selected from the group Cx40, Cx43, and Cx45;
a catheter, said catheter having a distal tip portion for engaging the cells of said cardiac tissue and delivering thereto said recombinant nucleic acid vectors;
a reservoir means comprising an inner chamber of said catheter for holding said recombinant nucleic acid vectors, said reservoir means located proximal to the said distal tip portion of said catheter; and
a delivery means for delivering and transfecting or transducing a therapeutically effective amount of said recombinant nucleic acid vectors from said reservoir means through said distal tip portion of said catheter to said cardiac tissue such that said cardiac tissue is transfected or transduced with said recombinant nucleic acid vectors;
wherein said catheter additionally comprises a mapping electrode means, a pacing electrode means, and a conductor means for connecting said mapping electrode means and a pacing electrode means to the proximal end of said catheter; and
wherein said mapping electrode means provides the means of determining the location of said cardiac tissue deficient in connexin expression for transfection or transducing said cells in said cardiac tissue and wherein said pacing electrode means provides means for testing or controlling the effect of the expression of said recombinant nucleic acid vectors with a pacemaker.
2. An implantable delivery system of claim 1 , wherein said distal tip portion of said catheter comprises a hollow helical element for engaging said cells of said cardiac tissue.
3. An implantable delivery system of claim 1 , wherein engaging said cells in said cardiac tissue comprises contacting the AV nodal tissue.
4. An implantable delivery system of claim 1 , wherein engaging said cells in said cardiac tissue comprises contacting the His tissue and bundle branches.