Cultures, products and methods using umbilical cord matrix cells
View Patent ↗Stem cells from human sources can have a variety of useful applications in disease treatment and biotechnology. More particularly the umbilical cord matrix cell cultures of the invention have a variety of totipotent, pluripotent, or multipotent cells for a variety of end uses from a non-controversial, universally available, species-specific source. The technology can have application to any amniotic animal, including agricultural and laboratory animals and humans. The invention relates to isolating the stem cells, culturing the stem cells, maintaining the stem cells, transforming the stem cells into useful cell types using genetic or other transformation technologies, stem cell and tissue banking and using untransformed or transformed cells in disease treatment.
1. A method for obtaining a population of cells from an umbilical cord matrix comprising:
a) enzymatically dispersing umbilical cord matrix to provide enzymatically dispersed umbilical cord matrix cells;
b) culturing the enzymatically dispersed umbilical cord matrix cells in the presence of epidermal growth factor (EGF) and platelet derived growth factor (PDGF) to proliferate the umbilical cord matrix cells;
c) culturing the enzymatically dispersed umbilical cord matrix cells on a substrate surface and removing non-adherent cells;
d) culturing adherent cells from c) to select for a population of umbilical cord matrix cells that comprise cells that are negative for CD34 and CD45, positive for telomerase activity, can be expanded in vitro, and maintained in culture through repeated passages.
2. A population of umbilical cord matrix cells isolated by
a) enzymatically dispersing umbilical cord matrix to provide enzymatically dispersed umbilical cord matrix cells;
b) culturing the enzymatically dispersed umbilical cord matrix cells in the presence of epidermal growth factor (EGF) and platelet derived growth factor (PDGF) to proliferate the umbilical cord matrix cells;
c) culturing the enzymatically dispersed umbilical cord matrix cells on a substrate surface and removing non-adherent cells;
d) culturing adherent cells from (c) to select for a population of umbilical cord matrix cells that comprise cells that are negative for CD34 and CD45, positive for telomerase activity, can be expanded in vitro, and maintained in culture through repeated passages.
3. A method of banking a population of umbilical cord matrix cells comprising providing a population of cells accordingly to claim 2 and banking the cells.
4. The method of claim 3 , further comprising tissue typing said population of umbilical cord matrix cells.
5. The method of claim 3 , further comprising expanding said population of umbilical cord matrix cells.
6. The method of claim 3 further comprising passaging the population of umbilical cord matrix cells for at least 10 times, wherein the umbilical cord matrix cells remain stable during said passaging.
7. The method of claim 3 wherein the umbilical cord matrix cells are human cells.
8. The method of claim 3 wherein the umbilical cord matrix cells are porcine or bovine cells.
9. The method of claim 3 wherein the umbilical cord matrix stem cells are equine or canine cells.
10. The method of claim 3 wherein the umbilical cord matrix stem cells are rodent cells.
11. An umbilical cord matrix cell culture comprising an umbilical cord matrix cell population according to claim 2 and a feeder cell population.
12. The cell culture of claim 11 wherein the umbilical cord matrix cell population is typed, banked or expanded.
13. The cell culture of claim 12 wherein the umbilical cord matrix cell population and the feeder cells are of human origin.