BSL2v1c2 polypeptides
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polypeptide comprising a sequence selected from the group consisting of:
(a) an isolated polypeptide comprising amino acids 1 to 316 of SEQ ID NO:13;
(b) an isolated polypeptide comprising amino acids 2 to 316 of SEQ ID NO:13; and
(c) an isolated polypeptide comprising amino acids 29 to 316 of SEQ ID NO:13.
2. The isolated polypeptide of claim 1 , wherein said polypeptide is (a).
3. The isolated polypeptide of claim 1 , wherein said polypeptide is (b).
4. The isolated polypeptide of claim 1 , wherein said polypeptide is (c).
5. An isolated polypeptide comprising a polypeptide encoded by the cDNA clone contained in plasmid BSL2-L165-35b in ATCC Deposit No. PTA-1988.
6. An isolated polypeptide produced by a method comprising:
(a) culturing an isolated recombinant host cell comprising a vector that comprises the coding region encoding the polypeptide of claim 1 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
7. The isolated polypeptide of claim 1 wherein said polypeptide further comprises a heterologous polypeptide.
8. The isolated polypeptide of claim 7 wherein said heterologous polypeptide is the Fc domain of human IgG.
9. An isolated polypeptide comprising a polypeptide encoded by the cDNA clone contained in plasmid BSL2v1c2Ig in ATCC Deposit No. PTA-4056.
10. An isolated polypeptide comprising a sequence selected from the group consisting of:
(a) an isolated polypeptide comprising amino acids 1 to 247 of SEQ ID NO:13;
(b) an isolated polypeptide comprising amino acids 2 to 247 of SEQ ID NO:13; and
(c) an isolated polypeptide comprising amino acids 29 to 247 of SEQ ID NO:13.
11. The isolated polypeptide of claim 10 , wherein said polypeptide is (a).
12. The isolated polypeptide of claim 10 , wherein said polypeptide is (b).
13. The isolated polypeptide of claim 10 , wherein said polypeptide is (c).