Test strip for creatinine determination
The present invention provides a novel test piece for creatinine measurement. The test piece includes a compound expressed by the following formula (1), a metal that forms a colored complex with the compound, and a buffer agent in a porous material. The amount of creatinine is determined by optically measuring a colored complex of the compound and the metal and evaluating the degree of inhibition of the colored complex formation by creatinine. In the formula (1), R 1 represents H, SO 3 X, or COOX. R 4 and R 6 represent OH, SO 3 X, or COOX and may be either the same or different. R 2 , R 3 , R 5 , and R 7 represent H, OH, Cl, Br, I, NO 2 , NO, or CH 3 and may be either the same or different. Xs in the R 1 , R 4 , and R 6 represent H, Na, K, or NH 4 and may be either the same or different.
1. A test piece for creatinine measurement comprising:
a compound expressed by the following formula (1)
where R 1 represents H, SO 3 X or COOX,
R 4 and R 6 represent OH, SO 3 X, or COOX and are either the same or different,
R 2 , R 3 , R 5 and R 7 represent H, OH, Cl, Br, I, NO 2 , NO, or CH 3 and are either the same or different, and
Xs in the R 1 , R 4 and R 6 represent H, Na, K, or NH 4 and are either the same or different, and
a transition metal or its salt that forms a colored complex with the compound,
wherein the transition metal is Pd(II), wherein the compound and the transition metal are included in a porous material.
2. The test piece for creatinine measurement according to claim 1 , wherein the compound is expressed by the following formula (2)
where R 1 represents H, SO 3 X, or COOX,
R 4 and R 6 represent OH, SO 3 X, or COOX and are either the same or different,
R 2 , R 3 , R 5 and R 7 represent H, OH, Cl, Br, I, NO 2 , NO, or CH 3 and are either the same or different, and
Xs in the R 1 , R 4 and R 6 represent H, Na, K, or NH 4 and are either the same or different.
3. The test piece for creatinine measurement according to claim 2 , wherein the compound is expressed by the following formula (3)
where R 1 represents H, SO 3 X, or COOX,
R 2 and R 3 represent H, OH, Cl, Br, I, NO 2 , NO, or CH 3 and are either the same or different, and
Xs represent H, Na, K, or NH 4 and are either the same or different.
4. The test piece for creatinine measurement according to claim 1 , wherein the compound is expressed by the following formula (4)
5. The test piece for creatinine measurement according to claim 1 , wherein the compound is expressed by the following formula (5)
6. The test piece for creatinine measurement according to claim 1 , wherein the compound is expressed by the following formula (6)
7. The test piece for creatinine measurement according to claim 1 , wherein the compound is expressed by the following formula (7)
8. The test piece for creatinine measurement according to claim 1 , wherein the compound (A) and the metal or its salt (B) are present at a ratio (molar ratio A:B) of 30:1 to 1:15.
9. The test piece for creatinine measurement according to claim 1 , further comprising a buffer agent.
10. The test piece for creatinine measurement according to claim 9 , wherein the compound (A) and the buffer agent (C) are present at a ratio (molar ratio A:C) of 1:10 to 1:1000.
11. The test piece for creatinine measurement according to claim 1 , further comprising a surfactant.
12. The test piece for creatinine measurement according to claim 11 , wherein the compound (A) and the surfactant (D) are present at a ratio (molar ratio A:D) of 50:1 to 3:1.