Method for forming an optimally exposed image of cytological specimen
First and second images of respective portions of a cytological specimen are acquired at different brightness levels. Sections of the respective first image are selected and combined with selected sections of the second image of each portion to form a composite image, so that non-nuclei cytological components that would otherwise appear dark in a single exposure image are brighter in the composite image.
1. A method of generating a substantially optimally exposed image of a cytological specimen, comprising:
acquiring a first image of the specimen at a first brightness level;
acquiring a second image of the specimen at a second brightness level higher than the first brightness level;
combining the respective sections of the first and second images to form a substantially optimally exposed image of the cytological specimen;
selecting sections of the first image which correspond to non-nuclei components of the cytological specimen acquired at the first brightness level; and
selecting sections of the second image corresponding to nuclei components of the cytological specimen acquired at the second brightness level.
2. The method of claim 1 , wherein the first and second images are obtained of substantially a same portion of the specimen, the specimen portion containing both nuclei and non-nuclei components of individual cells.
3. The method of claim 2 , wherein nuclei components of the individual cells are more distinct in the second image than in the first image.
4. The method of claim 2 , wherein non-nuclei components of the individual cells are more distinct in the first image than in the second image.
5. The method of claim 1 , wherein the first brightness level is based on a first exposure time, and the second brightness level is based on a second exposure time different than the first exposure time.
6. The method of claim 1 , wherein the second brightness level is about four times greater than the first brightness level.
7. The method of claim 1 , wherein the first brightness level is a non-saturation brightness level, and the second brightness level is a saturation brightness level.
8. The method of claim 1 , wherein the respective selected sections of the first and second images are combining by replacing pixels of the selected sections of one of the first and second images with pixels of the selected sections of the other one of the first and second images.
9. The method of claim 1 , wherein the respective selected sections of the first and second images are combining by merging pixels of the selected sections of the first image with pixels of the selected sections of the second image.
10. The method of claim 1 , further comprising generating a mask that includes pixels of the first image representing nuclei and non-nuclei components of the specimen.
11. The method of claim 10 , wherein the mask is generated by filtering and binarizing the first image, the mask including binary values representing both nuclei and non-nuclei cells components.
12. A method of generating a substantially optimally exposed image of a cytological specimen, comprising:
acquiring a first image of the specimen at a first brightness level;
acquiring a second image of the specimen at a second brightness level higher than the first brightness level; and
combining the respective sections of the first and second images to form a substantially optimally exposed image of the cytological specimen,
wherein the first image containing cell nuclei having a gray scale brightness value of approximately 20, non-nuclei cell components having a grayscale brightness value in a range of approximately 50-150, and background having a gray scale brightness of about 229, respectively, based on a gray scale brightness range of 0-255.
13. The method of claim 12 , the second image containing cell nuclei having a gray scale brightness value of approximately 80, and non-nuclei cell components and background that are saturated, respectively, based on the gray scale brightness range of 0-255.
14. A method of generating a substantially optimally exposed image of a cytological specimen, comprising:
acquiring a first image of a first portion of the specimen, the first image being acquired at a first brightness level;
acquiring a second image of the first portion of the cytological specimen, the second image being acquired at a second brightness level that is higher than the first brightness level;
selecting sections of the first image corresponding to non-nuclei components of the first portion of the specimen;
selecting sections of the second image corresponding to the nuclei components of the first portion of the specimen; and
combining the respective selected sections of the first and second images to form an substantially optimally exposed image of the first portion of the specimen.
15. The method of claim 14 , wherein the second brightness level is about four times higher than the first brightness level.
16. The method of claim 14 , wherein the first brightness level is a non-saturation brightness level, and the second brightness level is a saturation brightness level.
17. The method of claim 14 , wherein nuclei components of the individual cells are more distinct in the second image than in the first image, and wherein non-nuclei components of the individual cells are more distinct in the first image than in the second image.
18. The method of claim 14 , wherein the respective selected sections of each of the respective first and second images are combining by merging pixels of the selected sections of the first image with pixels of the selected sections of the second image.