Sirtuin modulating compounds
Provided herein are novel sirtuin-modulating compounds and methods of use thereof. The sirtuin-modulating compounds may be used for increasing the lifespan of a cell, and treating and/or preventing a wide variety of diseases and disorders including, for example, diseases or disorders related to aging or stress, diabetes, obesity, neurodegenerative diseases, cardiovascular disease, blood clotting disorders, inflammation, cancer, and/or flushing as well as diseases or disorders that would benfit from increased mitochondrial activity. Also provided are compositions comprising a sirtuin-modulating compound in combination with another therapeutic agent.
1. A compound of the formula:
or a salt thereof, wherein:
each of X 7 , X 8 , X 9 and X 10 is independently selected from N, CR 20 , or CR 1 ′, wherein:
each R 20 is independently selected from H or a solubilizing group;
each R 1 ′ is independently selected from H or optionally substituted C 1 -C 3 straight or branched alkyl, wherein when R 1 ′ is substituted, R 1 ′ is substituted with one or more of —OH, halogen, —OR a , —O—COR a , —COR a , —C(O)R a , —CN, —NO 2 , —COOH, —COOR a , —OCO 2 R a , —C(O)NR a R b , —OC(O)NR a R b , —SO 3 H, —NH 2 , —NHR a , —N(R a R b ), —COOR a , —CHO, —CONH 2 , —CONHR a , —CON(R a R b ), —NHCOR a , —NRCOR a , —NHCONH 2 , —NHCONR a H, —NHCON(R a R b ), —NR c CONH 2 , —NR c CONR a H, —NR c CON(R a R b ), —C(═NH)—NH 2 , —C(═NH)—NHR a , —C(═NH)—N(R a R b ), —C(═NR c )—NH 2 , —C(═NR c )—NHR a , —C(═NR c )—N(R a R b ), —NH—C(═NH)—NH 2 , —NH—C(═NH)—NHR a , —NH—C(═NH)—N(R a R b ), —NH—C(═NR c )—NH 2 , —NH—C(═NR c )—NHR a , —NH—C(═NR c )—N(R a R b ), —NR d H—C(═NH)—NH 2 , —NR d —C(═NH)—NHR a , —NR d —C(═NH)—N(R a R b ), —NR d —C(═NR c )—NH 2 , —NR d —C(═NR c )—NHR a , —NR d —C(═NR c )—N(R a R b ), —NHNH 2 , —NHNHR a , —NHR a R b , —SO 2 NH 2 —SO 2 NHR a , —SO 2 NR a R b , —CH═CHR a , —CH═CR a R b , —CR c ═CR a R b , CR c ═CHR a , —CR c ═CR a R b , —CCR a , —SH, —SO k R a , —S(O) k OR a and —NH—C(═NH)—NH 2 , wherein
k is 0, 1 or 2;
R a —R d are each independently an aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group; and
—NR a R b , taken together, can also form a substituted or unsubstituted non-aromatic heterocyclic group;
wherein a non-aromatic heterocyclic group, benzylic group or aryl group can also have an aliphatic or substituted aliphatic group as a substituent; a substituted aliphatic group can also have a non-aromatic heterocyclic ring, a substituted non-aromatic heterocyclic ring, benzyl, substituted benzyl, aryl or substituted aryl group as a substituent; and a substituted aliphatic, non-aromatic heterocyclic group, substituted aryl, or substituted benzyl group can have more than one substituent;
one of X 7 , X 8 , X 9 and X 10 is N and the others are selected from CR 20 or CR 1 ′; and
zero to one R 20 is a solubilizing group;
R 19 is selected from:
wherein:
each Z 10 , Z 11 , Z 12 and Z 13 is independently selected from N, CR 20 , or CR 1 ′; and
each Z 14 , Z 15 and Z 16 is independently selected from N, NR 1 ′, S, O, CR 20 , or CR 1 ′,
wherein:
zero to two of Z 10 , Z 11 , Z 12 and Z 13 are N;
at least one of Z 14 , Z 15 and Z 16 is N, NR 1 ′, S or O;
zero to one of Z 14 , Z 15 and Z 16 is S or O;
zero to two of Z 14 , Z 15 and Z 16 are N or NR 1 ′;
zero to one R 20 is a solubilizing group;
zero to one R 1 ′ is an optionally substituted C 1 -C 3 straight or branched alkyl; and
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 ′—S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(O)—O— or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from an optionally substituted monocyclic or bicyclic aryl, or an optionally substituted monocyclic or bicyclic heteroaryl, with the proviso that:
when X 7 is N, R 19 is
and each of Z 10 , Z 11 , Z 12 and Z 13 is independently selected from CR 20 or CR 1 ′, then:
a) at least one of X 8 , X 9 and X 10 is C—(C 1 -C 3 straight or branched alkyl) or C-(solubilizing group); or
b) at least one of Z 10 , Z 11 , Z 12 and Z 13 is CR 20 , wherein R 20 is a solubilizing group.
2. A compound of the formula:
or a salt thereof, wherein:
R 1 ′ is selected from H or optionally substituted C 1 -C 3 straight or branched alkyl, wherein when R 1 ′ is substituted, R 1 ′ is substituted with one or more of —OH, halogen, —OR a , —O—COR a , —COR a , —C(O)R a , —CN, —NO 2 , —COOH, —COOR a , —OCO 2 R a , —C(O)NR a R b , —OC(O)NR a R b , —SO 3 H, —NH 2 , —NHR a , —N(R a R b ), —COOR a , —CHO, —CONH 2 , —CONHR a , —CON(R a R b ), —NHCOR a , —NRCOR a , —NHCONH 2 , —NHCONR a H, —NHCON(R a R b ), —NR c CONH 2 , —NR c CONR a H, —NR c CON(R a R b ), —C(═NH)—NH 2 , —C(═NH)—NHR a , —C(═NH)—N(R a R b ), —C(═NR c )—NH 2 , —C(═NR c )—NHR a , —C(═NR c )—N(R a R b ), —NH—C(═NH)—NH 2 , —NH—C(═NH)—NHR a , —NH—C(═NH)—N(R a R b ), —NH—C(═NR c )—NH 2 , —NH—C(═NR c )—NHR a , —NH—C(═NR c )—N(R a R b ), —NR d H—C(═NH)—NH 2 , —NR d —C(═NH)—NHR a , —NR d —C(═NH)—N(R a R b ), —NR d —C(═NR c )—NH 2 , —NR d —C(═NR c )—NHR a , —NR d —C(═NR c )—N(R a R b ), —NHNH 2 , —NHNHR a , —NHR a R b , —SO 2 NH 2 —SO 2 NHR a , —SO 2 NR a R b , —CH═CHR a , —CH═CR a R b , —CR c ═CR a R b , CR c ═CHR a , —CR c ═CR a R b , —CCR a , —SH, —SOR a —S(O) k OR a and —NH—C═NH, —NH 2 , wherein
k is 0, 1 or 2;
R a -R d are each independently an aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group; and
—NR a R b , taken together, can also form a substituted or unsubstituted non-aromatic heterocyclic group;
wherein a non-aromatic heterocyclic group, benzylic group or aryl group can also have an aliphatic or substituted aliphatic group as a substituent; a substituted aliphatic group can also have a non-aromatic heterocyclic ring, a substituted non-aromatic heterocyclic ring, benzyl, substituted benzyl, aryl or substituted aryl group as a substituent; and a substituted aliphatic, non-aromatic heterocyclic group, substituted aryl, or substituted benzyl group can have more than one substituent;
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 ′—S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R′ 1 —CR 1 ′R′ 1 ′, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R′ 1 —CR 1 ′R′ 1 ′, —NR 1 ′—C(O)—O— or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from an optionally substituted monocyclic or bicyclic aryl, or an optionally substituted monocyclic or bicyclic heteroaryl, with the provisos that:
when R 21 is —NH—C(O)—, R 31 is not unsubstituted furyl, 5-bromofuryl, unsubstituted phenyl, phenyl monosubstituted with halo or methyl, 3- or 4-methoxyphenyl, 4-butoxyphenyl, 4-t-butylphenyl, 3-trifluoromethylphenyl, 2-benzoylphenyl, 2- or 4-ethoxyphenyl, 2,3-, 2,4-, 3,4-, or 3,5-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 2,4- or 2,6-difluorophenyl, 3,4-dioxymethylene phenyl, 3,4- or 3,5-dimethylphenyl, 2-chloro-5-bromophenyl, 2-methoxy-5-chlorophenyl, unsubstituted quinolinyl, thiazolyl substituted simultaneously with methyl and phenyl, or ethoxy-substituted pyridinyl;
when R 21 is —NH—C(O)—CH(CH 2 —CH 3 )—, R 31 is not unsubstituted phenyl;
when R 21 is —NH—C(O)—CH 2 —, R 31 is not unsubstituted phenyl, 3-methylphenyl, 4-chlorophenyl, 4-ethoxyphenyl, 4-fluorophenyl or 4-methoxyphenyl;
when R 21 is —NH—C(O)—CH 2 —O—, R 31 is not unsubstituted phenyl or 4-chlorophenyl; and
when R 21 is —NH—S(O) 2 —, R 31 is not 3,4-dioxymethylene phenyl, 2,4,5-trimethylphenyl, 2,4,6-trimethylphenyl, 2,4- or 3,4-dimethylphenyl, 2,5-difluorophenyl, 2,5- or 3,4-dimethoxyphenyl, fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-ethylphenyl, 4-methylphenyl, 3-methyl-4-methoxyphenyl, unsubstituted phenyl, unsubstituted pyridinyl, unsubstituted thienyl, chloro-substituted thienyl, or methyl-substituted benzothiazolyl.
3. A composition comprising a compound of the formula:
or a salt thereof, wherein:
each of X 7 , X 8 , X 9 and X 10 is independently selected from N, CR 20 , or CR 1 ′, wherein:
each R 20 is independently selected from H or a solubilizing group;
each R 1 ′ is independently selected from H or optionally substituted C 1 -C 3 straight or branched alkyl, wherein when R 1 ′ is substituted, R 1 ′ is substituted with one or more of —OH, halogen, —OR a , —O—COR a , —COR a , —C(O)R a , —CN, —NO 2 , —COOH, —COOR a , —OCO 2 R a , —C(O)NR a R b , —OC(O)NR a R b , —SO 3 H, —NH 2 , —NHR a , —N(R a R b ), —COOR a , —CHO, —CONH 2 , —CONHR a , —CON(R a R b ), —NHCOR a , —NRCOR a , —NHCONH 2 , —NHCONR a H, —NHCON(R a R b ), —NR c CONH 2 , NR c CONR a H, —NR c CON(R a R b ), —C(═NH)—NH 2 , —C(═NH)—NHR a , —C(═NH)—N(R a R b ), —C(═NR c )—NH 2 , —C(═NR c )—NHR a , —C(═NR c )—N(R a R b ), —NH—C(═NH)—NH 2 , —NH—C(═NH)—NHR a , —NH—C(═NH)—N(R a R b ), —NH—C(═NR c )—NH 2 , —NH—C(═NR c )—NHR a , —NH—C(═NR c )—N(R a R b ), —NR d H—C(═NH)—NH 2 , —NR d —C(═NH)—NHR a , —NR d- C(═NH)—N(R a R b ), —NR d —C(═NR c )—NH 2 , —NR d —C(═NR c )—NHR a , —NR d —C(═NR c )—N(R a R b ), —NHNH 2 , —NHNHR a , —NHR a R b , —SO 2 NH 2 —SO 2 NHR a , —SO 2 NR a R b —CH═CHR a , —CH═CR a R b , —CW═CR a R b , CR c ═CHR a , —CR a ═CR a R b , —CCR a , —SH, —SO k R a , —S(O) k C a and —NH—C(═NH)—NH 2 , wherein
k is 0, 1 or 2;
R a -R d are each independently an aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group; and
—NR a R b , taken together, can also form a substituted or unsubstituted non-aromatic heterocyclic group;
wherein a non-aromatic heterocyclic group, benzylic group or aryl group can also have an aliphatic or substituted aliphatic group as a substituent; a substituted aliphatic group can also have a non-aromatic heterocyclic ring, a substituted non-aromatic heterocyclic ring, benzyl, substituted benzyl, aryl or substituted aryl group as a substituent; and a substituted aliphatic, non-aromatic heterocyclic group, substituted aryl, or substituted benzyl group can have more than one substituent;
one of X 7 , X 8 , X 9 and X 10 is N and the others are selected from CR 20 or CR 1 ′; and
zero to one R 20 is a solubilizing group;
R 19 is selected from:
wherein:
each Z 10 , Z 11 , Z 12 and Z 13 is independently selected from N, CR 20 , or CR 1 ′; and
each Z 14 , Z 15 and Z 16 is independently selected from N, NR 1 ′, S, O, CR 20 , or CR 1 ′,
wherein:
zero to two of Z 10 , Z 11 , Z 12 and Z 13 are N;
at least one of Z 14 , Z 15 and Z 16 is N, NR 1 ′, O or S;
zero to one of Z 14 , Z 15 and Z 16 is S or O;
zero to two of Z 14 , Z 15 and Z 16 are N or NR 1 ′;
zero to one R 20 is a solubilizing group;
zero to one R 1 ′ is an optionally substituted C 1 -C 3 straight or branched alkyl;
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 ′—S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′—, CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R′ 1 —CR 1 ′R′ 1 ′, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R′ 1 —CR 1 ′R′ 1 ′, —NR 1 ′—C(O)—O— or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from an optionally substituted monocyclic or bicyclic aryl, or an optionally substituted monocyclic or bicyclic heteroaryl,
with the proviso that when R 19 is
Z 10 , Z 11 , Z 12 and Z 13 are each CH, and R 21 is —NHC(O)—, R 31 is not an optionally substituted phenyl, wherein the composition is pyrogen-free.
4. A pharmaceutical composition comprising:
a. a compound of the formula:
or a pharmaceutically acceptable salt thereof, wherein:
each of X 7 , X 8 , X 9 and X 10 is independently selected from N, CR 20 , or CR 1 ′, wherein:
each R 20 is independently selected from H or a solubilizing group;
each R 1 ′ is independently selected from H or optionally substituted C 1 -C 3 straight or branched alkyl, wherein when R 1 ′ is substituted, R 1 ′ is substituted with one or more of —OH, halogen, —OR a , —O—COR a , —COR a , —C(O)R a , —CN, —NO 2 , —COOH, —COOR a , —OCO 2 R a , —C(O)NR a R b , —OC(O)NR a R b , —SO 3 H, —NH 2 , —NHR a , —N(R a R b ), —COOR a , —CHO, —CONH 2 , —CONHR a , —CON(R a R b ), —NHCOR a , —NRCOR a , —NHCONH 2 , —NHCONR a H, —NHCON(R a R b ), —NR c CONH 2 , NR c CONR a H, —NR c CON(R a R b ), —C(═NH)—NH 2 , —C(═NH)—NHR a , —C(═NH)—N(R a R b ), —C(═NR c )—NH 2 , —C(═NR c )—NHR a , —C(═NR c )—N(R a R b ), —NH—C(═NH)—NH 2 , —NH—C(═NH)—NHR a , —NH—C(═NH)—N(R a R b ), —NH—C(═NR c )—NH 2 , —NH—C(═NR c )—NHR a , —NH—C(═NR c )—N(R a R b ), —NR d H—C(═NH)—NH 2 , —NR d —C(═NH)—NHR a , —NR d —C(═NH)—N(R a R b ), —NR d —C(═NR c )—NH 2 , —NR d —C(═NR c )—NHR a , —NR d —C(═NR c )—N(R a R b ), —NHNH 2 , —NHNHR a , —NHR a R b , —SO 2 NH 2 —SO 2 NHR a , —SO 2 NR a R b , —CH═CHR a , —CH═CR a R b , —CR c ═CR a R b , CR c ═CHR a , —CR c ═CR a R b , —CCR a , —SH, —SO k R a , —S(O) k OR a and —NH—C(═NH)—NH 2 , wherein
k is 0, 1 or 2;
R a —R d are each independently an aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group; and
—NR a R b , taken together, can also form a substituted or unsubstituted non-aromatic heterocyclic group;
wherein a non-aromatic heterocyclic group, benzylic group or aryl group can also have an aliphatic or substituted aliphatic group as a substituent; a substituted aliphatic group can also have a non-aromatic heterocyclic ring, a substituted non-aromatic heterocyclic ring, benzyl, substituted benzyl, aryl or substituted aryl group as a substituent; and a substituted aliphatic, non-aromatic heterocyclic group, substituted aryl, or substituted benzyl group can have more than one substituent;
one of X 7 , X 8 , X 9 and X 10 is N and the others are selected from CR 20 or CR 1 ′; and
zero to one R 20 is a solubilizing group;
R 19 is selected from:
wherein:
each Z 10 , Z 11 , Z 12 and Z 13 is independently selected from N, CR 20 , or CR 1 ′; and
each Z 14 , Z 15 and Z 16 is independently selected from N, NR 1 ′, S, O, CR 20 , or CR 1 ′,
wherein:
zero to two of Z 10 , Z 11 , Z 12 and Z 13 are N;
at least one of Z 14 , Z 15 and Z 16 is N, NR 1′, 0 or S;
zero to one of Z 14 , Z 15 and Z 16 is S or O;
zero to two of Z 14 , Z 15 and Z 16 are N or NR 1 ′;
zero to one R 20 is a solubilizing group;
zero to one R 1 ′ is an optionally substituted C 1 -C 3 straight or branched alkyl;
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 —S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′—, CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(O)—O— or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from an optionally substituted monocyclic or bicyclic aryl, or an optionally substituted monocyclic or bicyclic heteroaryl,
with the proviso that when R 19 is
Z 10 , Z 11 , Z 12 and Z 13 are each CH, and R 21 is —NHC(O)—, R 31 is not an optionally substituted phenyl; and
b. a pharmaceutically acceptable carrier or diluent.
5. A packaged pharmaceutical comprising a compound of the formula:
or a pharmaceutically acceptable salt thereof, wherein:
each of X 7 , X 8 , X 9 and X 10 is independently selected from N, CR 20 , or CR 1 ′, wherein:
each R 20 is independently selected from H or a solubilizing group;
each R 1 ′ is independently selected from H or optionally substituted C 1 -C 3 straight or branched alkyl, wherein when R 1 ′ is substituted, R 1 ′ is substituted with one or more of —OH, halogen, —OR a , —O—COR a , —COR a , —C(O)R a , —CN, —NO 2 , —COOH, —COOR a , —OCO 2 R a , —C(O)NR a R b , —OC(O)NR a R b , —SO 3 H, —NH 2 , —NHR a , —N(R a R b ), —COOR a , —CHO, —CONH 2 , —CONHR a , —CON(R a R b ), —NHCOR a , —NRCOR a , —NHCONH 2 , —NHCONR a H, —NHCON(R a R b ), —NR c CONH 2 , NR c CONR a H, —NR c CON(R a R b ), —C(═NH)—NH 2 , —C(═NH)—NHR a , —C(═NH)—N(R a R b ), —C(═NR c )—NH 2 , —C(═NR c )—NHR a , —C(═NR c )—N(R a R b ), —NH—C(═NH)—NH 2 , —NH—C(═NH)—NHR a , —NH—C(═NH)—N(R a R b ), —NH—C(═NR c )—NH 2 , —NH—C(═NR c )—NHR a , —NH—C(═NR c )—N(R a R b ), —NR d H—C(═NH)—NH 2 —, —NR d —C(═NH)—NHR a , —NR d —C(═NH)—N(R a R b ), —NR d —C(═NR c )—NH 2 , —NR d —C(═NR c )—NHR a , —NR d —C(═NR c )—N(R a R b ), —NHNH 2 , —NHNHR a , —NHR a R b , —SO 2 NH 2 —SO 2 NHR a , —SO 2 NR a R b , —CH═CHR a , —CH═CR a R b , —CR═CR a R b , CR c ═CHR a , —CR═CR a R b , —CCR a , —SH, —SO k R a , —S(O) k OR a and —NH—C(═NH)—NH 2 , wherein
k is 0, 1 or 2;
R a -R d are each independently an aliphatic, substituted aliphatic, benzyl, substituted benzyl, aromatic or substituted aromatic group; and
—NR a R b , taken together, can also form a substituted or unsubstituted non-aromatic heterocyclic group;
wherein a non-aromatic heterocyclic group, benzylic group or aryl group can also have an aliphatic or substituted aliphatic group as a substituent; a substituted aliphatic group can also have a non-aromatic heterocyclic ring, a substituted non-aromatic heterocyclic ring, benzyl, substituted benzyl, aryl or substituted aryl group as a substituent; and a substituted aliphatic, non-aromatic heterocyclic group, substituted aryl, or substituted benzyl group can have more than one substituent;
one of X 7 , X 8 , X 9 and X 10 is N and the others are selected from CR 20 or CR 1 ′; and
zero to one R 20 is a solubilizing group;
R 19 is selected from:
wherein:
each Z 10 , Z 11 , Z 12 and Z 13 is independently selected from N, CR 20 , or CR 1 ′; and
each Z 14 , Z 15 and Z 16 is independently selected from N, NR 1 ′, S, O, CR 20 , or CR 1 ′,
wherein:
zero to two of Z 10 , Z 11 , Z 12 and Z 13 are N;
at least one of Z 14 , Z 15 and Z 16 is N, NR 1 ′, O or S;
zero to one of Z 14 , Z 15 and Z 16 is S or O;
zero to two of Z 14 , Z 15 and Z 16 are N or NR 1 ′;
zero to one R 20 is a solubilizing group;
zero to one R 1 ′ is an optionally substituted C 1 -C 3 straight or branched alkyl;
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 ′—S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′—CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R′ 1 —CR 1 ′R′ 1 —, —NR 1 ′—C(O)—O— or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from an optionally substituted monocyclic or bicyclic aryl, or an optionally substituted monocyclic or bicyclic heteroaryl,
with the proviso that when R 19 is
Z 10 , Z 11 , Z 12 and Z 13 are each CH, and R 21 is —NHC(O)—, R 31 is not an optionally substituted phenyl and instructions for using the compound to modulate a sirtuin.
6. The compound according to claim 1 , wherein R 19 is selected from optionally substituted phenyl, optionally substituted pyridyl, optionally substituted thienyl or optionally substituted furyl.
7. The compound according to claim 1 , wherein:
R 19 is
wherein each of Z 10 , Z 11 , Z 12 and Z 13 is independently selected from CR 20 or CRC; and
R 21 is selected from —NH—C(O)—, —NH—C(O)—CH(CH 3 )—O—, —NH—C(O)—CH 2 —O—, or —NH—S(O) 2 —CH 2 —CH 2 —; and
R 31 is selected from an optionally substituted aryl, or an optionally substituted heteroaryl other than phenyl-substituted furyl.
8. The compound according to claim 7 , wherein R 31 is optionally substituted with 1 to 3 substituents independently selected from —OCH 3 , —CH 3 , —N(CH 3 ) 2 , phenyl, phenoxy, 3,4-dioxymethylene, fluoro, or another solubilizing group.
9. The compound according to claim 7 , wherein R 31 is selected from unsubstituted quinolinyl, 2,4-dimethoxyphenyl, 3,4-dimethoxyphenyl, 3,5-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 2,3,4-trimethoxyphenyl, 2-dimethylaminophenyl, 3-dimethylaminophenyl, 4-dimethylaminophenyl, 3,5-dimethylphenyl, 3,5-difluorophenyl, 3-trifluoromethoxyphenyl, unsubstituted quinoxalinyl, unsubstituted benzopyrimidinyl,
10. The compound according to claim 1 , wherein R 19 is selected from
each of Z 10 , Z 11 , Z 12 and Z 13 is independently selected from CR 20 , or CRC;
R 21 is selected from —NH—C(O)—, NH—C(O)—CH 2 —CH(CH 3 )—O, —NH—C(O)—NH—, —NH—C(S)—NH—, —NH—C(S)—NH—CH 2 —, or —NH—S(O) 2 —;
R 31 is selected from an optionally substituted phenyl, an optionally substituted naphthyl, or an optionally substituted heteroaryl, wherein:
when X 8 is N, R 21 is —NH—C(S)—NH—, and R 19 is phenyl, R 31 is not 2-methoxy-5-nitrophenyl, 2-S-methylphenyl or 2-acetylphenyl;
when X 8 is N, R 21 is —NH—S(O) 2 —, and R 19 is phenyl, R 31 is not thiadiazole-substituted thienyl or 4-methylsulfonylphenyl;
when X 8 is N, R 21 is —NH—CO—, and R 19 is phenyl, R 31 is not 2,4-difluorophenyl, pyridyl-substituted thienyl, 3,4-dichlorophenyl, 4-t-butylphenyl, or 3-benzyloxyphenyl;
when X 9 is N, R 21 is —NH—C(O)— and R 19 is
R 31 is not 2,3,4-trimethoxyphenyl or 3,5-dimethoxyphenyl; and
when X 9 is N, R 21 is —NH—C(O)— and R 19 is phenyl, R 31 is not 3,5-dimethoxyphenyl.
11. The compound according to claim 10 , wherein R 31 is selected from phenyl, naphthyl, pyrazolyl, furyl, thienyl, pyridyl, isoxazolyl, benzopyrazolyl, benzofuranyl, benzothienyl, quinolinyl, benzoisoxazolyl, or
and wherein R 31 is optionally substituted.
12. The compound according to claim 11 , wherein R 31 is optionally substituted with up to three substituents independently selected from —OCH 3 , —CH 3 , —N(CH 3 ) 2 , —O-phenyl, or another solubilizing group.
13. The compound according to claim 11 , wherein R 31 is selected from unsubstituted phenyl, 3-methoxyphenyl, 4-methoxyphenyl, 2,3 dimethoxyphenyl, 2,4-dimethoxyphenyl, 2,5-bis(trifluoromethyl)phenyl, 3,4-dimethoxyphenyl, 3,5-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 2,3,4-trimethoxyphenyl, 2-methoxy-4-methylphenyl, 2-phenoxyphenyl, 3-dimethylaminophenyl, 4-dimethylaminophenyl, unsubstituted 2-furanyl, unsubstituted 2-thienyl,
14. The compound according to claim 2 , wherein:
R 1 ′ is selected from H or optionally substituted C 1 -C 3 straight or branched alkyl;
R 21 is selected from —NR 1 ′—C(O)—, —NR 1 ′—S(O) 2 —, —NR 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—, —NR 1 ′—C(S)—NR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—NR 1 ′—, —NR 1 ′—C(═NR 1 ′)—NR 1 ′—, —C(O)—NR 1 ′—, —C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—, —CR 1 ′R 1 ′—, —NR 1 ′—C(O)—CR 1 ′═CR 1 ′—, —NR 1 ′—S(O) 2 —NR 1 ′—, —NR 1 ′—C(O)—NR 1 ′—S(O) 2 —, —NR 1 ′—CR 1 ′R 1 ′—C(O)—NR 1 ′—, —CR 1 ′R 1 ′—C(O)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′—CR 1 ′—CR 1 ′R 1 ′—, —NR 1 ′—C(═N—CN)—NR 1 ′—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—O—, —NR 1 ′—C(O)—CR 1 ′R 1 ′—CR 1 ′R 1 ′—O—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—, —NR 1 ′—S(O) 2 —CR 1 ′R 1 ′—CR 1 ′R 1 ′—, or —NR 1 ′—C(O)—CR 1 ′R 1 ′—; and
R 31 is selected from a monocyclic or bicyclic aryl or a monocyclic or bicyclic heteroaryl, and comprises a solubilizing group substituent.
15. The compound according to claim 2 , wherein R 31 is selected from phenyl, naphthyl, pyrazolyl, furyl, thienyl, pyridyl, isoxazolyl, benzopyrazolyl, benzofuranyl, benzothienyl, quinolinyl, benzoisoxazolyl, or
and wherein R 31 is optionally substituted.
16. The compound according to claim 2 , wherein:
R 21 is selected from —NH—C(O)—, NH—C(O)—CH 2 —CH(CH 3 )—O, —NH—C(O)—NH—, —NH—C(S)—NH—, —NH—C(S)—NH—CH 2 —, or —NH—S(O) 2 —; and
R 31 is selected from an optionally substituted phenyl, an optionally substituted naphthyl, or an optionally substituted heteroaryl.
17. The compound according to claim 16 , wherein R 31 is selected from unsubstituted phenyl, 3-methoxyphenyl, 4-methoxyphenyl, 2,3 dimethoxyphenyl, 2,4-dimethoxyphenyl, 2,5-bis(trifluoromethyl)phenyl, 3,4-dimethoxyphenyl, 3,5-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 2,3,4-trimethoxyphenyl, 2-methoxy-4-methylphenyl, 2-phenoxyphenyl, 3-dimethylaminophenyl, 4-dimethylaminophenyl, unsubstituted 2-furanyl, unsubstituted 2-thienyl,
18. The compound according to claim 1 , wherein R 21 is —NH—C(O)—.
19. The compound according to claim 2 , wherein R 21 is —NH—C(O)—.
20. The composition according to claim 3 , wherein R 21 is —NH—C(O)—.
21. The pharmaceutical composition according to claim 4 , wherein R 21 is —NH—C(O)—.
22. The packaged pharmaceutical according to claim 5 , wherein R 21 is —NH—C(O)—.
23. A pharmaceutical composition comprising a pharmaceutically acceptable carrier or diluent and a compound of any of claims 1 , and 6 - 13 , and 18 .