Proteins with an attached short peptide of acidic amino acids
View Patent ↗Disclosed are a fusion protein comprising enzyme N-acetylgalactosamine-6-sulfate sulfatase and a short peptide consisting of 4-15 acidic amino acids attached to the enzyme on its N-terminal side, a pharmaceutical composition containing the fusion protein, and a method for treatment of type A Morquio disease using the fusion protein. Compared with the native enzyme protein, the fusion protein exhibits higher transferability to bone tissues and improved, higher stability in the blood.
1. A fusion protein consisting of SEQ ID NO: 7.
2. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a fusion protein consisting of SEQ ID NO: 7.
3. The fusion protein according to claim 1 , which has increased stability in the blood and enhanced transferability from the blood to bone tissues compared with native N-acetylgalactosamine-6-sulfate sulfatase.
4. The pharmaceutical composition according to claim 2 , wherein said fusion protein has increased stability in the blood and enhanced transferability from the blood to bone tissues compared with native N-acetylgalactosamine-6-sulfate sulfatase.
5. A fusion protein consisting of amino acids 20-528 of SEQ ID NO: 7.
6. The fusion protein according to claim 1 , which has been purified.
7. The fusion protein according to claim 1 , which has enhanced in vivo stability compared to native GALNS.