Centrifuge system utilizing disposable components and automated processing of blood to collect platelet rich plasma
A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.
1. An apparatus for processing blood comprising:
a centrifuge assembly including a centrifuge, a separation container and a drive assembly for rotating the centrifuge about a rotation axis;
flexible tubing for transferring blood between a first container to the separation container and for transferring a separated fluid from the separation container to a collection container; and
a loop device for defining a loop in the flexible tubing, the loop device having an arm assembly including a latch for mating with and supporting a tube clamp and an arm for positioning the latch a distance from the centrifuge, the latch being positioned such that the tube clamp is positioned on the rotation axis of the centrifuge.
2. The apparatus of claim 1 , wherein the first container is a syringe.
3. The apparatus of claim 1 , wherein the second container is a syringe.
4. The apparatus of claim 1 , wherein the loop device includes contact surfaces configured for reduced wear of portions of the flexible tubing abutting the contact surfaces.
5. The system of claim 1 , wherein the separation container includes two sections separated by a divider element that includes a vent that is in communication with both sections, wherein each section includes a cylindrical elongated portion adjacent the divider element, a collection portion with a side wall that slopes inward toward the central axis of the section to define a reducing circular cross-section along the central axis, a cylindrical nipple attached to the collection portion, and a port fluidly connected to the flexible tubing.