Corn event DAS-59122-7 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. A method of detecting the presence of DNA corresponding to the DAS-59122-7 event in a sample, the method comprising:
(a) contacting a sample comprising maize DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from maize event DAS-59122-7 and a genomic region flanking said event and does not hybridize under said stringent hybridization conditions with a non-DAS-59122-7 maize plant DNA, wherein said probe hybridizes with i) SEQ ID NO. 24 or the complement thereof and ii) SEQ ID No. 19, SEQ ID NO. 20, or the complement of SEQ ID NO. 19 or 20, wherein i) and ii) are contiguous with one another
(b) subjecting the sample and probe to stringent hybridization conditions; and
(c) detecting hybridization of the probe to the DNA, wherein detection of hybridization indicates the presence of the DAS-59122-7 event.