IP Library Granted Patent US 7,892,813
Granted Patent B2
US 7,892,813 · App. 12/029,797 · Granted Feb 22, 2011

Fungal endophytes of

Assignee: The Samuel Roberts Noble Foundation
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Quick Facts
Patent No.
US 7,892,813
App. No.
12/029,797
Granted
Feb 22, 2011
Kind
B2
Abstract

The invention provides an isolated culture of a Neotyphodium endophyte of an Elymus canadensis host plant, wherein the endophyte reproduces asexually and enhances the agronomic characteristics of the host plant. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

Claims (24)

1. An isolated Neotyphodium sp, endophyte selected from the group consisting of: NFE1000, NFE1001, and NFE1002, cultures of the endophyte having been deposited with NRRL accession numbers NRRL 50005, NRRL 50004, and NRRL 50003, respectively.

2. A synthetic combination of the endophyte of claim 1 and a host plant.

3. The synthetic combination of claim 2 , wherein said host plant is a host grass plant.

4. The synthetic combination according to claim 3 , wherein:

a) the host grass plant displays at least equivalent reproductive vigor as a host grass plant identical except for the absence of the endophyte, when compared under identical conditions;

b) the endophyte protects the host grass plant from biotic and/or abiotic stresses; and

c) the host grass plant or a parental generation of the host grass plant is artificially inoculated with the endophyte.

5. The synthetic combination of claim 2 , wherein the host plant is an Elymus canadensis host plant.

6. The synthetic combination of claim 4 , wherein the synthetic combination is achieved by introduction of the endophyte to the host grass plant by a method selected from the group consisting of: inoculation, infection, grafting, and seed transmission.

7. The synthetic combination of claim 4 wherein the abiotic stress is selected from the group consisting of: water deficiency, nutrient deficiency, heat stress, salt toxicity, aluminum toxicity, and freezing temperatures.

8. The synthetic combination of claim 4 wherein the biotic stress is selected from the group consisting of: insect infestation, nematode infestation, and herbivore grazing.

9. Seed of a host grass plant comprising a synthetic combination of the endophyte of claim 1 with said host grass plant.

10. A method for propagating an Elymus canadensis - Neotyphodium sp. combination, comprising:

a obtaining a Elymus canadensis host grass plant comprising the synthetic combination of claim 5 ; and

b harvesting seed from the plant; the plant or a parental generation of the plant having been inoculated with said endophyte, or said endophyte having been introduced into a parental generation of the plant through crossing and/or backcrossing procedures.

11. A method for enhancing the growth or reproduction of an Elymus canadensis host grass plant in the presence of a biotic or abiotic stress, comprising: contacting the host grass plant with a strain of endophyte of Neotyphodium sp., selected from the group consisting of: NFE1000, NFE1001, and NFE1002, cultures of the endophyte having been deposited with NRRL accession numbers: NRRL 50005, NRRL 50004, and NRRL 50003, respectively, such that the endophyte colonizes the plant.

12. The method of claim 11 , wherein the host grass plant has enhanced root growth, more tillers, enhanced total biomass, or enhanced seed yield in comparison to an otherwise identical host grass plant lacking said endophyte.

13. The method of claim 11 , wherein the stress is a biotic stress caused by at least one organism selected from the group consisting of an herbivore, a nematode, and an insect.

14. The method of claim 13 , wherein the insect to which increased resistance is conferred on the host grass plant is selected from the group consisting of: fall armyworm and Russian wheat aphid.

15. The method of claim 11 , wherein colonization of the host grass plant is achieved by introduction of the endophyte to the host grass plant by a method selected from the group consisting of: inoculation, infection, grafting, seed transmission, and combinations thereof.

16. The method of claim 11 , wherein said biotic stress is a pest stress or an insect stress.

17. A method for propagating an Elymus canadensis - Neotyphodium sp. combination, comprising:

vegetatively reproducing Elymus canadensis plant tissue colonized by the synthetic combination of claim 5 .

18. The method of claim 11 , wherein said abiotic stress is a water deficit stress.

Assignments (2)
CHANGE OF NAME Recorded May 2, 2018
From: THE SAMUEL ROBERTS NOBLE FOUNDATION, INC.
To: NOBLE RESEARCH INSTITUTE, LLC
Reel/Frame 046054/0855 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 14, 2008
From: YOUNG, CAROLYN; HOPKINS, ANDY
To: THE SAMUEL ROBERTS NOBLE FOUNDATION
Reel/Frame 020946/0505 →
Continuity (2)
Provisional Application 60889480 · Feb 12, 2007
Related Publication 20080229441A1 · Sep 18, 2008