Synthesis of nitrodibenzylfuran chromophore for photodeprotection of organic molecules
View Patent ↗Inventors have developed a chromophore (nitrodibenzylfuranyl, or NBDF) for ultra efficient uncaging of a caged substrate (e.g., an organic molecule such as, for example, an amino acid, a biological molecules, such as, for example, second messengers inside cells). Photolysis of a NBDF derivative of EGTA (i.e. caged calcium) is about 50 times more efficient than others calcium cages (the quantum yield of photolysis is 0.6 and the extinction coefficient is 18,400. NDBF-EGTA has a 2-photon cross section of about 0.3-0.6 GM).
1. A photolabile compound represented by one of the following formulas:
NDBF methylbromide;
2. A photolabile compound represented by one of the following formulas:
3. A photolabile compound represented by one of the following formulas:
4. A photolabile compound represented by one of the following formulas:
5. A photolabile compound represented by one of the following formulas:
6. A method of photochemical release of an effector molecule from a caged compound, the method comprising:
(a) providing a photolabile compound of claim 4 ; and
(b) photolyzing the photolabile compound with light to release the effector molecule, wherein the effector molecule is selected from gamma-aminobutyric acid and glutamate.