Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
Preparations of novel molecular transporter compositions and their use for transporting bioactive substances into cells in living animals are disclosed. To afford in vivo delivery, the composition is covalently linked to the bioactive substance and the resultant composite structure is introduced into the subject. The transporter composition includes multiple guanidine moieties on a dendrimeric scaffold having a triazine core.
1. A composition structured for entry into cells, which may be in a living subject, comprising:
a) a triazine ring;
b) a dendrimeric structure containing 4 to 6 guanidine groups covalently linked to a carbon of the triazine ring;
c) a dendrimeric structure containing 4 to 6 guanidine groups covalently linked to a second carbon of the triazine ring; and
d) a bioactive substance covalently linked to a third carbon of the triazine ring,
wherein, the composition has the structure:
2. A composition structured for entry into cells, which may be in a living subject, comprising:
a) a triazine ring;
b) a dendrimeric structure containing 4 to 6 guanidine groups covalently linked to a carbon of the triazine ring;
c) a dendrimeric structure containing 4 to 6 guanidine groups covalently linked to a second carbon of the triazine ring; and
d) a bioactive substance covalently liked to a third carbon of the triazine ring,
wherein, the linkage between the triazine ring and the bioactive substance is readily cleaved within a cell and has the structure:
3. The composition of claim 1 , wherein the bioactive substance is a Morpholino antisense oligo.
4. The composition of claim 2 , wherein the bioactive substance is a Morpholino antisense oligo.
5. The composition of claim 3 , wherein the Morpholino antisense oligo is effective to modify splicing of a selected mRNA.
6. The composition of claim 4 , wherein the Morpholino antisense oligo is effective to modify splicing of a selected mRNA.
7. The composition of claim 5 , wherein the Morpholino antisense oligo is effective to correct a splicing error in the mRNA transcript that codes for a green fluorescent protein.