G-CSF-albumin fusion proteins
The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention.
1. An albumin fusion protein comprising a G-CSF polypeptide fused to an albumin polypeptide wherein said albumin fusion protein is produced from a host cell comprising Construct ID 1643.
2. The albumin fusion protein of claim 1 , which is glycosylated.
3. The albumin fusion protein of claim 1 , which is non-glycosylated.
4. The albumin fusion protein of claim 1 , wherein said host cell is a yeast cell.
5. The albumin fusion protein of claim 4 , wherein said yeast is Saccharomyces cerevisiae.
6. The albumin fusion protein of claim 4 , wherein said yeast is glycosylation deficient.
7. The albumin fusion protein of claim 4 , wherein said yeast is glycosylation and protease deficient.
8. The albumin fusion protein of claim 1 , wherein said host cell is a mammalian cell.
9. The albumin fusion protein of claim 8 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
10. A composition comprising the albumin fusion protein of claim 1 and a pharmaceutically acceptable carrier.
11. A nucleic acid molecule comprising a polynucleotide encoding the albumin fusion protein of claim 1 .
12. The nucleic acid molecule of claim 11 , which encodes the albumin fusion protein further comprising an N-terminal methionine.
13. The nucleic acid molecule of claim 11 , which encodes the albumin fusion protein further comprising a signal sequence.
14. The nucleic acid molecule of claim 11 , which comprises at least one heterologous polynucleotide.
15. The nucleic acid molecule of claim 14 , wherein said heterologous polynucleotide is at least one selected from a vector sequence, a promoter sequence, a selectable marker, and a region for termination of transcription.
16. The nucleic acid molecule of claim 11 , which is part of an expression cassette.
17. A vector comprising the nucleic acid molecule of claim 11 .
18. An isolated host cell comprising the nucleic acid molecule of claim 11 .
19. An isolated host cell comprising the vector of claim 17 .
20. A method of producing an albumin fusion protein comprising:
(a) culturing the host cell of claim 18 in conditions such that the albumin fusion protein is expressed; and
(b) recovering the albumin fusion protein.
21. The albumin fusion protein produced by the method of claim 20 .
22. An albumin fusion protein comprising a G-CSF polypeptide fused to an albumin polypeptide, wherein said albumin fusion protein is produced from a host cell comprising the construct contained in ATCC Deposit No. PTA-3766.
23. The albumin fusion protein of claim 22 , which is glycosylated.
24. The albumin fusion protein of claim 22 , which is non-glycosylated.
25. The albumin fusion protein of claim 22 , wherein said host cell is a yeast cell.
26. The albumin fusion protein of claim 25 , wherein said yeast is Saccharomyces cerevisiae.
27. The albumin fusion protein of claim 25 , wherein said yeast is glycosylation deficient.
28. The albumin fusion protein of claim 25 , wherein said yeast is glycosylation and protease deficient.
29. The albumin fusion protein of claim 22 , wherein said host cell is a mammalian cell.
30. The albumin fusion protein of claim 29 , wherein said mammalian cell is a COS cell, a CHO cell, or a NSO cell.
31. The albumin fusion protein of claim 22 and a pharmaceutically acceptable carrier.
32. A nucleic acid molecule comprising a polynucleotide encoding the albumin fusion protein of claim 22 .
33. The nucleic acid molecule of claim 32 , which encodes the albumin fusion protein further comprising an N-terminal methionine.
34. The nucleic acid molecule of claim 32 , which encodes the albumin fusion protein further comprising a signal sequence.
35. The nucleic acid molecule of claim 32 , which comprises at least one heterologous polynucleotide.
36. The nucleic acid molecule of claim 35 , wherein said heterologous polynucleotide is at least one selected from a vector sequence, a promoter sequence, a selectable marker, and a region for termination of transcription.
37. The nucleic acid molecule of claim 32 , which is part of an expression cassette.
38. A vector comprising the nucleic acid molecule of claim 32 .
39. An isolated host cell comprising the nucleic acid molecule of claim 32 .
40. An isolated host cell comprising the vector of claim 38 .
41. A method of producing an albumin fusion protein comprising:
(a) culturing the host cell of claim 39 in conditions such that the albumin fusion protein is expressed; and
(b) recovering the albumin fusion protein.
42. The albumin fusion protein produced by the method of claim 41 .