Treating malignant tumors with high field strength electroporation of plasmids encoding IL-12

1. A method of treating cancer, the method comprising:

injecting an established cancerous tumor with an effective dose of plasmid coding for IL-12; and

administering electroporation therapy to the established cancerous tumor, the electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.

2. The method of claim 1 , wherein the established cancerous tumor is melanoma.

3. The method of claim 1 , wherein the at least one pulse delivered to the tumor is about 1500V/cm.

4. The method of claim 1 , wherein the duration of the at least one pulse is about 100 microseconds.

5. A method of treating cancer in a subject, the method comprising:

injecting an established cancerous tumor with an effective dose of plasmid coding for IL-12;

administering electroporation to the established cancerous tumor using six 1500V/cm pulses, each pulse being 100 microseconds in duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth;

injecting an effective dose of plasmid coding for IL-12 into the muscle tissue of the subject; and

administering electroporation to the subject intramuscularly using twelve 100V/cm pulses, each pulse being 20 milliseconds in duration.

6. The method of claim 5 , wherein the established cancerous tumor is melanoma.

7. A method of treating cancer, the method comprising:

administering a first treatment at time T1, wherein the first treatment comprises injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the tumor at time T1, the first electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond; and

administering a second treatment at time T2, wherein time T2 is a time later than time T1, wherein the second treatment comprises injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the tumor at time T2, the second electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.

8. The method of claim 7 , wherein the established cancerous tumor is melanoma.

9. The method of claim 7 , wherein the at least one pulse of the first electroporation therapy delivered to the established cancerous tumor and the at least one pulse of the second electroporation therapy delivered to the tumor are about 1500V/cm.

10. The method of claim 7 , wherein the duration of the at least one pulse of the first electroporation therapy delivered to the established cancerous tumor and the at least one pulse of the second electroporation therapy delivered to the established cancerous tumor are about 100 microseconds.

11. The method of claim 7 , wherein the duration between time T1 and T2 is seven days.

12. The method of claim 7 , further comprising:

administering a third treatment at time T3, wherein time T3 is a time later than time T2, wherein the third treatment comprises injecting the established cancerous tumor with a third effective dose of plasmid coding for IL-12 and administering a third electroporation therapy to the tumor, the third electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond.

13. The method of claim 7 , wherein the duration between time T1 and T2 is four days.

14. The method of claim 12 , wherein the duration between time T2 and T3 is three days.

15. A method of treating cancer, the method comprising:

administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration; and

administering a second treatment on day seven, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.

16. A method of treating cancer in a subject, the method comprising:

administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;

administering a second treatment on day seven, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth;

injecting an effective dose of plasmid coding for IL-12 into the muscle tissue of the subject; and

administering electroporation therapy to the subject intramuscularly using twelve pulses delivered at 100V/cm at 20 millisecond duration.

17. A method of treating cancer in a subject, the method comprising:

administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;

administering a second treatment on day four, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration; and

administering a third treatment on day seven, the third treatment comprising injecting the established cancerous tumor with a third effective dose of plasmid coding for IL-12 and administering a third electroporation therapy to the established cancerous tumor, the third electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.

18. The method of claim 17 , further comprising:

injecting an effective dose of plasmid coding for IL-12 into the muscle tissue of the subject; and

administering electroporation therapy to the subject intramuscularly using twelve pulses delivered at 100V/cm at 20 millisecond duration.