Methods and compositions for delivering polynucleotides
Methods and compositions for delivering polynucleotides are provided. One embodiment provides a non-viral vector comprising a recombinant polynucleotide-binding protein comprising a protein transduction domain operably linked to a targeting signal. Methods for modifying the genome of non-nuclear organelles are also provided.
1. A recombinant polynucleotide-packaging polypeptide comprising the amino acid sequence SEQ ID NO:210.
2. A fusion protein comprising a protein transduction domain fused to a mitochondrial localization signal fused to mature mitochondrial transcription factor A (TFAM).
3. The fusion protein of claim 2 wherein the mitochondrial localization signal is the mitochondrial localization signal of superoxide dismutase (Mn).
4. The fusion protein of claim 2 wherein the mature TFAM is mature human TFAM.
5. The fusion protein of claim 2 wherein the protein transduction domain comprises 8-15 positively charged amino acids.
6. The fusion protein of claim 2 wherein the protein transduction domain comprises 11 arginine residues.