Co-culture lymphoid tissue equivalent (LTE) for an artificial immune system (AIS)
View Patent ↗The present invention relates to methods for preparing an artificial immune system. The artificial immune system comprises a cell culture comprising T cells, B cells and antigen-primed dendritic cells. The artificial immune system of the present invention can be used for in vitro testing of vaccines, adjuvants, immunotherapy candidates, cosmetics, drugs, biologics and other chemicals.
1. A cell culture consisting of:
an enriched population of blood-derived T cells;
an enriched population of blood-derived B cells; and
dendritic cells primed with a selected antigen.
2. A cell culture consisting of:
an enriched population of blood-derived T cells;
an enriched population of blood-derived B cells; and
dendritic cells primed with a selected antigen; wherein the media of the cell culture is serum-free cell culture media.
3. A cell culture consisting of:
an enriched population of blood-derived T cells;
an enriched population of blood-derived B cells;
dendritic cells primed with a selected antigen; and
follicular dendritic cells.
4. A cell culture consisting of:
an enriched population of blood-derived T cells;
an enriched population of blood-derived B cells;
dendritic cells primed with a selected antigen; and
follicular dendritic cells; wherein the media of the cell culture is serum-free cell culture media.
5. The cell culture of any one of claims 1 - 4 , wherein the T cells and B cells are present in the cell culture in an approximately 1:1 ratio.
6. The cell culture of any one of claims 1 - 4 , wherein the T cells and B cells are present in the cell culture in a ratio between approximately 1:10 and approximately 10:1.
7. The cell culture of claim 2 or 4 , wherein the serum-free cell culture media is X-VIVO 15.
8. The cell culture of claim 4 , where the enriched population of T cells are CD4 + T cells.
9. The cell culture of claim 5 , where the enriched population of T cells are CD4 + T cells.
10. The cell culture of claim 6 , where the enriched population of T cells are CD4 + T cells.