Heteromeric taste receptors
Heteromeric taste receptors are provided. These receptors comprise a first polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R1 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R1 polypeptide or a different GPCR; and a second polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R3 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R3 polypeptide or that of a different GPCR.
1. A heteromeric taste receptor comprising:
(a) a first polypeptide which comprises extracellular and transmembrane domains wherein said transmembrane domains are at least 95% identical to the transmembrane domains of the T1R1 polypeptide of SEQ ID NO: 5, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of said T1R1 polypeptide; and
(b) a second polypeptide which comprises extracellular and transmembrane domains wherein said transmembrane domains are at least 95% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the T1R3 polypeptide.
2. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said first polypeptide are at least 96% identical to the transmembrane domains of the T1R1 polypeptide of SEQ ID NO: 5.
3. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said first polypeptide are is at least 97% identical to the transmembrane domains of the T1R1 polypeptide of SEQ ID NO: 5.
4. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said first polypeptide are at least 98% identical to the transmembrane domains of the T1R1 polypeptide of SEQ ID NO: 5.
5. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said first polypeptide are at least 99% identical to the transmembrane domains of the T1R1 polypeptide of SEQ ID NO: 5.
6. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said first polypeptide are identical to that of the T1R1 polypeptide of SEQ ID NO: 5.
7. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said second polypeptide are at least 96% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.
8. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said second polypeptide are at least 97% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.
9. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said second polypeptide are at least 98% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.
10. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said second polypeptide are at least 99% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.
11. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of said second polypeptide are identical to that of the T1R3 polypeptide of SEQ ID NO: 7.
12. The heteromeric taste receptor of claim 1 , wherein said heteromeric taste receptor is expressed in a eukaryotic cell.
13. The heteromeric taste receptor of claim 1 , wherein said heteromeric taste receptor is expressed in an HEK-293 cell.
14. The heteromeric taste receptor of claim 12 , wherein the cell contains a G protein.
15. The heteromeric taste receptor of claim 14 , wherein the G protein is Gα 15 or Gα 16 .