Method of preparing a treatment effective amount of allogeneic T-cells
Ex-vivo prepared T-cells are harvested from cell culture conditions and formulated in medium suitable for infusion. The formulation is made by labeling the cells with one or more agents which have reactivity for T-cell surface moieties capable of delivery activation signals upon cross-linking and mixing the labeled cells with biodegradable nanospheres or microspheres coated with a material capable of cross-linking the agents attached to the T-cell surface moieties. Alternatively, the formulation may be made by mixing a population of T-cells with biodegradable nanospheres or microspheres coated with a first material and one or more second materials. The first material binds the second material and the second material has reactivity for surface moieties on the T-cells and the interaction of the second materials with the T-cells causes the activation of the T-cells. In either method, the mixture of T-cells and biodegradable spheres are suspended in a medium suitable for infusion, and the mixture is packaged in a container.
1. A method for preparing a treatment effective amount of cells for infusion, of which a portion are allogeneic T-cells, the method comprising:
labeling the allogeneic T-cells with one or more agents which have reactivity for T-cell surface moieties capable of delivering activation signals upon cross-linking;
suspending the mixture of labeled allogeneic T-cells in an activated state with a cross-linking agent in a medium suitable for infusion;
packaging the mixture in a suitable container; and
administering the mixture to a patient allogeneically.
2. The method of claim 1 wherein the mixture is administered, applied, self-administered or self-applied subcutaneously, intramuscularly, intradermally, intravenously or intra-arterially.
3. The method of claim 1 wherein the container is a syringe or an IV infusion bag.
4. The method of claim 1 wherein the container is collapsible.
5. The method of claim 4 wherein the container includes opposing walls of flexible material and an exit tube.
6. A method for preparing a treatment effective amount of cells for infusion, of which a portion are allogeneic T-cells, the method comprising:
mixing a population of allogeneic T-cells with a first material and one or more second materials;
whereas the first material cross-links the second materials, and the second materials have reactivity for surface moieties on the T-cells, and whereas the interaction of the second materials with the T-cells causes the activation of the T-cells;
suspending the mixture of allogeneic T-cells in an activated state with a cross-linking agent in a medium suitable for infusion;
packaging the mixture in a container; and
administering parenterally the mixture to a patient allogeneically.
7. The method of claim 6 wherein the container is a syringe or an IV infusion bag.
8. The method of claim 6 wherein the container is collapsible.
9. The method of claim 8 wherein the container includes opposing walls of flexible material and an exit tube.
10. The method of claim 6 wherein the package mixture of the T-cell concentration of at least 1×10 7 cells per ml of infusion media.