Assay chip, and uses of said assay chip to determine molecular structures and functions

1. An assay chip for investigation of a functionality of non-lipid molecules and their interactions with molecules, comprising:

a nanopore substrate having a plurality of nanopores and alignment marks, said nanopore substrate having a thickness and said nonapores having a diameter in a range of 50 to 2000 nm resulting in an aspect ratio in a range of 0.5 to 2;

b) a substantially planar support layer deposited on said nanopore substrate and having a plurality of nanopores corresponding to and aligned with said nanopores of said nanopore substrate;

c) a biologically effective layer configured to host at least one of a non-lipid molecule and functional molecule, deposited on said support layer and covering the plurality of nanopores, resulting in accessible nanopores from both sides of the biologically effective layer for measurements, wherein the biologically effective layer is a biomembrane isolated from one of prokaryotic and eukaryotic cells, wherein the biologically effective layer is a lipid bilayer formed by preparation and later fusion of lipid vesicles or is a functional layer of supramolecular assembly, and said biologically effective layer retaining biological functionality.

2. The assay chip according to claim 1 , wherein a surface of the support layer is chemically modified by at least one of activated hydrophobic and hydrophilic silanes resulting in a support promotion layer.

3. The assay chip according to claim 1 , wherein the support layer is selected from the group consisting of silicon nitride (Si 3 N 4 ) and silicon oxide substrate, and wherein the substrate is selected from the group consisting of silicon and carbon containing materials, polymers, metals, dielectrica, glass and ceramics.

4. The assay chip according to claim 1 , wherein said nanopores are arranged in a plurality of nanopore array sections having an area in the range of 1×10 −6 mm 2 to 1 mm 2 on a total free standing silicon nitride membrane area of 1×10 −6 mm 2 to 10 mm 2 .

5. The assay chip according to claim 1 , wherein said nanopores have a distance from each other in the range of 0.5 to 5-times of their diameter.

6. The assay chip according to claim 5 , wherein said nanopores have a distance from each other in the range of 0.8 to 2-times of their diameter.

7. The assay chip according to claim 1 , wherein the non-lipid molecules are from a natural source selected from the group consisting of eukaryotes and prokaryotes.

8. The assay chip according to claim 1 , wherein the biologically effective layer hosts a non-lipid molecule, and wherein the non-lipid molecule is a synthetic compound.

9. The assay chip according to claim 1 , wherein the biomembrane and the lipid bilayer each comprise at least one of a non-lipid and functional molecule, whereby the functional molecule is produced using one of recombinant DNA and RNA technologies.

10. The assay chip according to claim 1 , wherein the biologically effective layer is made from at least one intact living cell.

11. The assay chip according to claim 1 , wherein the diameter of the nanopores is chosen in order to result with an aspect ratio in the range of 0.75 to 2.

12. The assay chip according to claim 1 , wherein the diameter of the nanopores is in the range of 100 to 2000 nm.

13. A process for analyzing a functionality of at least one of a non-lipid molecule and functional molecule being integrated in a biologically effective layer of an assay chip of claim 1 , and a biologically effective layer the process, comprising:

applying a fluid containing a binding compound to one side of the biologically effective layer in order to allow binding compound to interact with the non-lipid molecule;

monitoring the response of the non-lipid molecule induced by at least one effector binding and an interaction of binding molecules in the biologically effective layer by measuring physical or chemical changes on cis- or trans-sides of the assay chip.