Microorganisms and methods for conversion of syngas and other carbon sources to useful products
A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.
1. A non-naturally occurring microbial organism, comprising an isopropanol pathway, said microbial organism comprising at least one exogenous nucleic acid encoding a polypeptide having succinyl-CoA:3-ketoacid-CoA transferase activity; wherein said polypeptide is expressed in said microbial organism in a sufficient amount to produce isopropanol.
2. The organism of claim 1 , wherein said polypeptide having succinyl-CoA:3-ketoacid-CoA transferase activity is encoded by one or more genes selected from the group consisting of HPAG1 — 0676, HPAG1 — 0677, ScoA, ScoB, OXCT1, and OXCT2.
3. The organism of claim 1 , wherein said microbial organism further comprises a polypeptide having acetoacetyl-CoA thiolase activity, a polypeptide having acetoacetate decarboxylase activity, and a polypeptide having isopropanol dehydrogenase activity.
4. The organism of claim 3 , wherein said polypeptide having acetoacetyl-CoA thiolase activity is encoded by a gene selected from the group consisting of atoB, thlA, thlB, and erg10.
5. The organism of claim 3 , wherein said polypeptide having acetoacetate decarboxylase activity is encoded by the gene adc.
6. The organism of claim 3 , wherein said polypeptide having isopropanol dehydrogenase activity is encoded by a gene selected from the group consisting of adh and ipdh.
7. The organism of claim 1 , wherein said microbial organism further comprises at least one of the following: a corrinoid protein, a corrinoid iron-sulfur protein, a nickel-protein assembly protein, a ferredoxin, a polypeptide having methyltetrahydrofolate:corrinoid protein methyltransferase activity, a polypeptide having acetyl-CoA synthase activity, a polypeptide having carbon monoxide dehydrogenase activity, a polypeptide having pyruvate ferredoxin oxidoreductase activity, or a polypeptide having hydrogenase activity.
8. The organism of claim 7 , wherein said corrinoid protein is encoded by a gene selected from the group consisting of mtaC, mtaC1, mtaC2, and mtaC3.
9. The organism of claim 7 , wherein said polypeptide having methyltetrahydrofolate:corrinoid protein methyltransferase activity is encoded by a gene selected from the group consisting of mtaA, and mtaA1, mtaA2.
10. The organism of claim 7 , wherein said polypeptide having methyltetrahydrofolate:corrinoid protein methyltransferase activity is encoded by the gene acsE.
11. The organism of claim 7 , wherein said corrinoid iron-sulfur protein is encoded by the gene acsD.
12. The organism of claim 7 , wherein said nickel-protein assembly protein is encoded by at least one gene selected from the group consisting of acsF and cooC.
13. The organism of claim 7 , wherein said ferredoxin is encoded by the gene orf7.
14. The organism of claim 7 , wherein said polypeptide having acetyl-CoA synthase activity is encoded by at least one gene selected from the group consisting of acsB and acsC.
15. The organism of claim 7 , wherein said polypeptide having carbon monoxide dehydrogenase activity is encoded by the gene acsA.
16. The organism of claim 7 , wherein said polypeptide having pyruvate ferredoxin oxidoreductase activity is encoded by a gene selected from the group consisting of por and ydbK.
17. The organism of claim 7 , wherein said polypeptide having hydrogenase activity is encoded by at least one gene selected from the group consisting of hypA, hypB, hypC, hypD, hypE, hypF, moth — 2175, moth — 2176, moth — 2177, moth — 2178, moth — 2179, moth — 2180, moth — 2181, hycA, hycB, hycC, hycD, hycE, hycF, hycG, hycH, hycI, hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, moth — 2182, moth — 2183, moth — 2184, moth — 2185, moth — 2186, moth — 2187, moth — 2188, moth — 2189, moth — 2190, moth — 2191, moth — 2192, moth — 0439, moth — 0440, moth — 0441, moth — 0442, moth — 0809, moth — 0810, moth — 0811, moth — 0812, moth — 0813, moth — 0814, moth — 0815, moth — 0816, moth — 1193, moth — 1194, moth — 1195, moth — 1196, moth — 1717, moth — 1718, moth — 1719, moth — 1883, moth — 1884, moth — 1885, moth — 1886, moth — 1887, moth — 1888, moth — 1452, moth — 1453, and moth — 1454.
18. The organism of claim 7 , further comprising the polypeptide AcsEps encoded by the gene acsEps.
19. The organism of claim 7 , further comprising at least one enzyme or polypeptide encoded by a gene selected from the group consisting of codh, codh-I, cooF, hypA, cooH, cooU, cooX, cooL, cooK, cooM, cooT, cooJ, and codh-II.
20. The organism of claim 3 , wherein said microbial organism further comprises at least one of the following: a corrinoid protein, a corrinoid iron-sulfur protein, a nickel-protein assembly protein, a ferredoxin, a polypeptide having methyltetrahydrofolate:corrinoid protein methyltransferase activity, a polypeptide having acetyl-CoA synthase activity, a polypeptide having carbon monoxide dehydrogenase activity, a polypeptide having pyruvate ferredoxin oxidoreductase activity, or a polypeptide having hydrogenase activity.