Valve for controlling the flow of steam and other fluids
Systems (e.g., bioprocessing systems), including methods and apparatus, for controlling the flow of a sterilizing agent (e.g., steam) and/or at least one fluid reagent by utilizing a pressure-responsive valve, such as a rupture valve and/or a swing valve.
1. A method of fluid transfer using a conduit assembly including a conduit defining a channel and also including a rupture valve dividing the channel into an inlet portion and an outlet portion, the rupture valve having an inlet side and an outlet side, the method comprising:
applying steam to the outlet portion of the channel with the rupture valve restricting entry of the steam into the inlet portion;
rupturing the rupture valve by increasing a pressure exerted on at least a portion of the inlet side of the rupture valve to create a passageway through the rupture valve; and
adding a fluid reagent to a receiver vessel connected to the conduit assembly, from a supply vessel containing the fluid reagent and through the passageway of the rupture valve.
2. The method of claim 1 , wherein the step of adding a fluid reagent includes a step of adding a fluid reagent that is substantially liquid.
3. The method of claim 1 , wherein the step of rupturing includes a step of exerting pressure on the rupture valve via gas disposed downstream of the fluid reagent in the inlet portion.
4. The method of claim 1 , wherein the step of rupturing includes a step of exerting pressure on the rupture valve directly with the fluid reagent.
5. The method of claim 1 , further comprising a step of connecting the conduit assembly to the receiver vessel.
6. The method of claim 5 , wherein the step of connecting includes a step of connecting the conduit assembly such that the receiver vessel is farther downstream from the inlet portion than the outlet portion.
7. The method of claim 5 , wherein the receiver vessel is a bioreactor, and wherein the step of adding a fluid reagent includes a step of adding a medium for growing biological cells.
8. The method of claim 1 , wherein the conduit is a main conduit, wherein the conduit assembly also includes an ancillary conduit that branches from the main conduit, further comprising a step of removing steam condensate from the main conduit via the ancillary conduit during the step of applying steam.
9. The method of claim 1 , wherein the conduit assembly includes a filter operatively connected to the conduit, further comprising a step of filtering the fluid reagent to remove microorganisms from the fluid reagent during the step of adding.
10. The method of claim 1 , further comprising a step of sterilizing the inlet portion of the channel prior to the step of applying steam.
11. A method of fluid transfer using a conduit assembly including a main conduit defining a channel and also including an ancillary conduit that branches from the main conduit, the conduit assembly further including a rupture valve dividing the channel into an inlet portion and an outlet portion, the method comprising:
applying steam to the outlet portion of the channel with the rupture valve restricting entry of the steam into the inlet portion;
removing steam condensate from the main conduit via the ancillary conduit during the step of applying steam; and
rupturing the rupture valve with pressure exerted on the rupture valve from the inlet portion to create a passageway through the rupture valve for fluid flow.
12. The method of claim 11 , further comprising a step of connecting the conduit assembly to a receiver vessel and a step of adding a fluid reagent to the receiver vessel through the rupture valve.
13. The method of claim 12 , wherein the step of connecting includes a step of connecting the conduit assembly such that the receiver vessel is farther downstream from the inlet portion than the outlet portion.
14. The method of claim 12 , wherein the receiver vessel is a bioreactor, and wherein the step of adding a fluid reagent includes a step of adding a medium for growing biological cells.
15. The method of claim 12 , further comprising (1) a step of connecting the conduit assembly to a supply vessel containing a fluid reagent, and (2) a step of adding the fluid reagent to the receiver vessel through the rupture valve after the step of rupturing.
16. The method of claim 15 , wherein the conduit assembly includes a filter operatively connected to the conduit, further comprising a step of filtering the fluid reagent upstream of the rupture valve in the conduit assembly.
17. The method of claim 11 , further comprising a step of sterilizing the conduit assembly prior to the step of applying steam.
18. A method of fluid transfer using a conduit assembly including a conduit defining a channel and also including a rupture valve dividing the channel into an inlet portion and an outlet portion, the rupture valve having an inlet side and an outlet side, the method comprising:
applying steam to the outlet portion of the channel with the rupture valve restricting entry of the steam into the inlet portion;
rupturing the rupture valve by increasing a pressure exerted on at least a portion of the inlet side of the rupture valve to create a passageway through the rupture valve; and
flowing a fluid reagent through the passageway of the rupture valve.
19. The method of claim 18 , wherein the step of flowing a fluid reagent includes a step of flowing a fluid reagent that is substantially liquid.
20. The method of claim 18 , further comprising a step of sterilizing the inlet portion of the channel prior to the step of applying steam.