Human milk oligosaccharides to promote growth of beneficial gut bacteria
View Patent ↗The present invention provides prebiotic and probiotic compositions containing human milk oligosaccharides and methods of use that selectively promote the growth of beneficial gut bacteria.
1. A synthetic prebiotic composition comprising a first, second and third purified oligosaccharide each of which naturally occur in human breast milk, wherein the first oligosaccharide has a mass/charge (m/z) ratio of about 732.3; the second oligosaccharide has a mass/charge (m/z) ratio of about 1243.4; and the third oligosaccharide has a mass/charge (m/z) ratio of about 1389.5,
wherein the m/z ratio is measured by matrix-assisted laser desorption/ionization (MALDI), and
wherein the composition is consumed at a higher rate by Bifidobacterium longum bv. infantis ( B. infantis ) compared to other Bifidobacterium strains.
2. The synthetic prebiotic composition of claim 1 , where in the first oligosaccharide is selected from the group consisting of Lacto-N-tetraose and Lacto-N-neotetraose; the second oligosaccharide is selected from the group consisting of Monofucosyllacto-N-hexaose, Isomeric Fucosylated Lacto-N-hexaose (1), Isomeric Fucosylated Lacto-N-hexaose (2), Isomeric Fucosylated Lacto-N-hexaose (3); and the third oligosaccharide is selected from the group consisting of Difucosyl-para-lacto-neohexaose, Difucosyl-para-lacto-hexaose, and Difucosyl lacto-hexaose.
3. The synthetic prebiotic composition of claim 1 , further comprising a fourth oligosaccharide that naturally occurs in human breast milk and has a mass/charge (m/z) ratio of about 1097.4.
4. The synthetic prebiotic composition of claim 3 , wherein the fourth oligosaccharide is selected from the group consisting of Lacto-N-hexaose, Lacto-N-neohexaose, Para-lacto-hexaose, and Para-lacto-neohexaose.
5. The synthetic prebiotic composition of claim 3 , further comprising a fifth oligosaccharide that naturally occurs in human breast milk and has a mass/charge (m/z) ratio of about 878.3.
6. The synthetic prebiotic composition of claim 5 , wherein the fifth oligosaccharide is selected from the group consisting of Lacto-N-fucopentaose I, Lacto-N-fucopentaose II, Lacto-N-fucopentaose III, and Lacto-N-fucopentaose IV.
7. The synthetic prebiotic composition of claim 1 , further comprising an inoculum of B. infantis.
8. The synthetic prebiotic composition of claim 1 , further comprising a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch.
9. The synthetic prebiotic composition of claim 1 , wherein said prebiotic composition is in the form of a powder, a tablet, a capsule, a lozenge, a chewing gum, a food product, or a supplemented beverage.
10. The synthetic prebiotic composition of claim 9 , wherein said supplemented beverage is a member selected from the group consisting of an infant formula, follow-on formula, toddler's beverage, milk, fruit juice, and fruit-based drink.
11. The synthetic prebiotic composition of claim 1 , wherein said first, second and third oligosaccharide are each at a concentration of 100 micrograms/L to 25 grams/L.
12. A synthetic prebiotic composition comprising at least three purified oligosaccharides that naturally occur in human breast milk, wherein the oligosaccharides have a mass/charge (m/z) ratio selected from the group consisting of about 878.3, about 1097.4, about 1243.4, about 1389.5, about 1462.5, about 1608.6, about 1754.6, about 1827.6, and about 1900.7,
wherein the m/z ratio is measured by matrix-assisted laser desorption/ionization (MALDI), and
wherein the composition is consumed at a higher rate by Bifidobacterium longum bv. infantis ( B. infantis ) compared to other Bifidobacterium strains.
13. The synthetic prebiotic composition of claim 12 , wherein the oligosaccharides are selected from the group consisting of Lacto-N-Tetraose, Lacto-N-Neotetraose, Lacto-N-Fucopentaose I, Lacto-N-Fucopentaose II, Lacto-N-Fucopentaose III, Lacto-N-Fucopentaose V, Lacto-N-Hexaose, Para-Lacto-N-Hexaose, Lacto-N-Neohexaose, Para-Lacto-N-Neohexaose, Monofucosyllacto-N-Hexaose II, Isomeric Fucosylated Lacto-N-Hexaose (1), Monofucosyllacto-N-Hexaose, Isomeric Fucosylated Lacto-N-Hexaose (3), Isomeric Fucosylated Lacto-N-Hexaose (2), Difucosyl-Para-Lacto-N-Neohexaose, Difucosyl-Para-Lacto-N-Hexaose, Difucosyllacto-N-Hexaose, Lacto-N-Neoocataose, Para-Lacto-N-Octanose, Iso-Lacto-N-Octaose, Lacto-N-Octaose, Monofucosyllacto-N-neoocataose, Monofucosyllacto-N-Ocataose, Difucosyllacto-N-Octaose I, Difucosyllacto-N-Octaose II, Difficosyllacto-N-Neoocataose II, Difucosyllacto-N-Neoocataose I, Lacto-N-Decaose, Trifucosyllacto-N-Neooctaose, Trifucosyllacto-N-Octaose and Trifucosyl-Iso-Lacto-N-Octaose.
14. The synthetic prebiotic of claim 12 or 13 , comprising at least two, at least three, at least four or at least five oligosaccharides selected from the group.
15. The synthetic prebiotic composition of claim 12 , further comprising an inoculum of B. infantis.
16. A method of producing a synthetic prebiotic food product or food supplement, the method comprising, combining at least one food ingredient with the first and second and third oligosaccharide of claim 1 , thereby generating a prebiotic food product or food supplement.
17. The method of claim 16 , wherein the food ingredient comprises a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch.
18. The method of claim 16 , further comprising combining an inoculum of B. infantis with the food ingredient and/or first and second and third oligosaccharide.
19. The method of claim 16 , wherein at least one of the first, second and third oligosaccharides are synthesized before the combining step.
20. The method of claim 16 , wherein at least one of the first, second and third oligosaccharides are purified before the combining step.