Method of fabricating lipid bilayer membranes on solid supports
View Patent ↗The present invention provides a method of producing a planar lipid bilayer on a solid support. With this method, a solution of lipid vesicles is first deposited on the solid support. Next, the lipid vesicles are destabilized by adding an amphipathic peptide solution to the lipid vesicle solution. This destabilization leads to production of a planar lipid bilayer on the solid support. The present invention also provides a supported planar lipid bilayer, where the planar lipid bilayer is made of naturally occurring lipids and the solid support is made of unmodified gold or titanium oxide. Preferably, the supported planar lipid bilayer is continuous. The planar lipid bilayer may be made of any naturally occurring lipid or mixture of lipids, including, but not limited to phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinsitol, cardiolipin, cholesterol, and sphingomyelin.
1. A supported planar lipid bilayer comprising:
a) a planar lipid bilayer comprising naturally occurring lipids; and
b) a solid support comprising unmodified gold or titanium oxide;
wherein said lipid bilayer is supported by said solid support.
2. The supported planar lipid bilayer as set forth in claim 1 , wherein said lipids are phospholipids.
3. The supported planar lipid bilayer as set forth in claim 1 , wherein said lipids are selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, cardiolipin, cholesterol, and sphingomyelin.
4. The supported planar lipid bilayer as set forth in claim 1 , wherein said planar lipid bilayer is continuous.