Nanoscale sensors
Various aspects of the invention relate to nanoscale wire devices and methods of use for detecting analytes. In one aspect, the invention relates to a nanoscale electrical sensor array device, comprising at least one n-doped semiconductor nanoscale wire and at least one p-doped semiconductor nanoscale wire, each having a reaction entity immobilized thereon. Binding of an analyte to the immobilized reaction entity causes a detectable change in the electrical property of the nanoscale wire. In some embodiments, the reaction entity can be a nucleic acid that may interact with other nucleic acids, proteins, etc. In a specific embodiment, the nucleic acid may interact with an enzyme such as telomerase, which can extend the nucleic acid. In other embodiments, the analyte to be detected can be a toxin, virus or small molecule. Systems and methods of using such nanoscale devices are also disclosed, for example, within a microarray.
1. A nanoscale electrical sensor array device, comprising:
a first, n-doped semiconductor nanoscale wire;
a second, p-doped semiconductor nanoscale wire differing in composition from the first nanoscale wire;
a first reaction entity immobilized relative to the first nanoscale wire such that a binding event involving the first reaction entity is detectable by the nanoscale electrical sensor array device, wherein the first reaction entity is an entity that can interact with an analyte to cause a detectable change in an electrical property of the first nanoscale wire; and
a second reaction entity, identical to the first reaction entity, immobilized relative to the second nanoscale wire such that a binding event involving the second reaction entity is detectable by the nanoscale electrical sensor array device independently of detection of a binding event involving the first reaction entity.
2. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity is able to bind a virus.
3. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity is an antibody.
4. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity is a ganglioside.
5. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity comprises a binding partner of an analyte.
6. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity is able to bind a nucleic acid.
7. The nanoscale electrical sensor array device of claim 1 , wherein the first reaction entity is able to bind a protein.
8. A method of detecting an analyte using a nanoscale wire, the method comprising:
exposing a first, n-doped semiconductor nanoscale wire and a second, p-doped semiconductor nanoscale wire to a sample suspected of containing an analyte, the first nanoscale wire having immobilized relative thereto a first reaction entity and the second nanoscale wire having immobilized relative thereto a second reaction entity identical to the first reaction entity; and
determining a first detectable change in an electrical property of the first nanoscale wire in response to a first binding event involving the first reaction entity and a second detectable change in an electrical property of the second nanoscale wire in response to a second binding event involving the second reaction entity, wherein, if the first detectable change and the second detectable change are opposite responses, the sample is determined as containing the analyte.
9. The method of claim 8 , wherein, if the first detectable change and the second detectable change are not opposite responses, then the sample is determined as not containing the analyte.
10. The method of claim 8 , wherein the first detectable change is conductance and the second detectable change is conductance.
11. The method of claim 8 , wherein the analyte is a virus.
12. The method of claim 8 , wherein the first reaction entity is an antibody, and the second reaction entity is an antibody.
13. The method of claim 8 , wherein the first reaction entity is a specific binding partner of the analyte.
14. The method of claim 8 , wherein the first reaction entity is a nucleic acid.
15. The method of claim 8 , wherein the first reaction entity is a protein.