Processing and analysis techniques involving in-vessel material generation
View Patent ↗In at least one embodiment, the inventive technology relates to in-vessel generation of a material from a solution of interest as part of a processing and/or analysis operation. Preferred embodiments of the in-vessel material generation (e.g., in-vessel solid material generation) include precipitation; in certain embodiments, analysis and/or processing of the solution of interest may include dissolution of the material, perhaps as part of a successive dissolution protocol using solvents of increasing ability to dissolve.
1. A non-chromatographic method comprising the steps of:
providing a vessel having a substantially chemically inert stationary phase established therein and having at least one vessel inlet;
inputting an aqueous, polar, precipitant solvent into said vessel through at least one vessel inlet;
inputting an aqueous solution into said vessel through at least one vessel inlet;
intentionally precipitating a material within said vessel and in the presence of said substantially chemically inert stationary phase, wherein said substantially chemically inert stationary phase is substantially chemically inert relative to said material;
generating a remnant liquid upon performing said step of intentionally precipitating said material;
inputting a material dissolving, aqueous solvent into said vessel through at least one vessel inlet; and
dissolving at least a portion of said material with said material dissolving, aqueous solvent to generate an aqueous, dissolved material solution,
wherein said step of intentionally precipitating a material comprises the step of intentionally precipitating a material selected from the group consisting of tannins, metals and proteins.
2. A non-chromatographic method as described in claim 1 wherein said step of inputting an aqueous solution into said vessel through at least one vessel inlet comprises the step of inputting a sample into said vessel.
3. A non-chromatographic method as described in claim 2 wherein said step of inputting a sample into said vessel through at least one vessel inlet comprises the step of inputting wine, water or fluids into said vessel.
4. A non-chromatographic method as described in claim 2 wherein said step of inputting an aqueous solution into said vessel through at least one vessel inlet further comprises the step of inputting a sample solvent into said vessel.
5. A non-chromatographic method as described in claim 2 wherein said substantially chemically inert stationary phase is also substantially chemically inert relative to said sample.
6. A non-chromatographic method as described in claim 1 wherein said step of inputting an aqueous solution into said vessel through at least one vessel inlet comprises the step of inputting wine, water or fluid into said vessel.
7. A non-chromatographic method as described in claim 1 wherein said step of inputting an aqueous, polar precipitant solvent into said vessel through at least one vessel inlet comprises the step of inputting into said an aqueous, highly polar, precipitant solvent.
8. A non-chromatographic method as described in claim 1 wherein said step of generating a remnant liquid comprises the step of generating a remnant solution.
9. A non-chromatographic method as described in claim 2 further comprising the step of determining at least one characteristic of said sample.
10. A non-chromatographic method as described in claim 9 wherein said step of determining at least one characteristic of said sample comprises the step of using a technique selected from the group consisting of evaporative light scattering, mass spectrometry, optical absorbance, x-ray, conductivity, oxidation/reduction, refractive index, polarimetry, atomic spectroscopy, and fluorescence.
11. A non-chromatographic method as described in claim 9 wherein said step of determining at least one characteristic of said sample comprises the step of analyzing said remnant liquid.
12. A non-chromatographic method as described in claim 9 wherein said step of determining at least one characteristic of said sample comprises the step of determining a mass percentage of said material.
13. A non-chromatographic method as described in claim 9 wherein said step of determining at least one characteristic of said sample comprises the step of analyzing said aqueous, dissolved material solution.
14. A non-chromatographic method as described in claim 1 wherein said material comprises fluid proteins.
15. A non-chromatographic method as described in claim 9 wherein said step of determining at least one characteristic of said sample comprises the step of determining at least two characteristics of said sample.
16. A non-chromatographic method as described in claim 1 further comprising the step of separating said remnant liquid from said material.
17. A non-chromatographic method as described in claim 1 further comprising the step of removing said remnant liquid from said vessel.
18. A non-chromatographic method as described in claim 16 further comprising the step of replacing said remnant liquid with said material dissolving, aqueous solvent.
19. A non-chromatographic method as described in claim 1 further comprising the step of eluting said aqueous, dissolved material solution from said vessel.
20. A non-chromatographic method as described in claim 1 wherein said step of dissolving at least a portion of said material with said material dissolving, aqueous solvent comprises the step of dissolving only a first portion of said material with said material dissolving, aqueous solvent.
21. A non-chromatographic method as described in claim 20 further comprising the step of inputting a second material dissolving, aqueous solvent into said vessel through at least one vessel inlet to dissolve at least a second portion of said material.
22. A non-chromatographic method as described in claim 21 wherein said step of inputting a second material dissolving, aqueous solvent into said vessel comprises the step of inputting a stronger material dissolving, aqueous solvent.
23. A non-chromatographic method as described in claim 22 wherein said step of inputting a stronger material dissolving, aqueous solvent into said vessel comprises the step of inputting into said vessel solvent that gradually increases in strength.
24. A non-chromatographic method as described in claim 23 wherein said step of inputting into said vessel solvent that gradually increases in strength is performed during continuous solvent flow.
25. A non-chromatographic method as described in claim 22 wherein said strengths of said material dissolving, aqueous solvents do not change in a step gradient fashion.
26. A non-chromatographic method as described in claim 22 wherein said strengths of said material dissolving, aqueous solvents change in a step gradient fashion.
27. A non-chromatographic method as described in claim 20 further comprising the step of inputting increasingly stronger material dissolving, aqueous solvent into said vessel to dissolve at least a second portion of said material and generate a second aqueous, dissolved material solution.
28. A non-chromatographic method as described in claim 27 wherein said step of inputting increasingly stronger material dissolving, aqueous solvent comprises the step of inputting material dissolving, aqueous solvent that gradually increases in strength.
29. A non-chromatographic method as described in claim 28 wherein said step of step of inputting material dissolving, aqueous solvent that gradually increases in strength is performed during continuous solvent flow.
30. A non-chromatographic method as described in claim 27 where input solvent strengths do not change in a step gradient fashion.
31. A non-chromatographic method as described in claim 21 further comprising the step of replacing said aqueous, dissolved material solution with said second material dissolving, aqueous solvent.
32. A non-chromatographic method as described in claim 27 further comprising the steps of analyzing said second aqueous, dissolved material solution.
33. A non-chromatographic method as described in claim 27 wherein said step of inputting increasingly stronger material dissolving, aqueous solvent into said vessel to dissolve at least a second portion of said material comprises the step of inputting increasingly stronger material dissolving, aqueous solvent into said vessel to dissolve said second and at least a third portion of said material.
34. A non-chromatographic method as described in claim 33 further comprising the step of generating a third aqueous, dissolved material solution.
35. A non-chromatographic method as described in claim 34 further comprising the step of analyzing said third aqueous, dissolved material solution.
36. A non-chromatographic method as described in claim 1 further comprising the step of fractionating said aqueous solution into at least two parts.
37. A non-chromatographic method as described in claim 1 wherein each of said steps is started in the order in which it appears.
38. A non-chromatographic method as described in claim 1 wherein said step of providing a vessel having a substantially chemically inert stationary phase established therein comprises the step of providing a vessel having established therein a stationary phase selected from the group of: oligomers of PTFE, polymers of PTFE, polyphenylene sulfide, fluorinated polymers, silicon polymer and PEEK.
39. A non-chromatographic method as described in claim 1 wherein said step of providing a vessel having a substantially chemically inert stationary phase established therein comprises the step of providing a column having a substantially chemically inert stationary phase established therein.
40. A non-chromatographic method as described in claim 39 wherein said step of providing a column comprises the step of providing a column that is part of a chromatograph.
41. A non-chromatographic method as described in claim 1 wherein said step of providing a vessel having a substantially chemically inert stationary phase established therein comprises the step of providing a batch type vessel having a substantially chemically inert stationary phase established therein.
42. A non-chromatographic method as described in claim 1 wherein said step of intentionally precipitating a material within said vessel comprises the step of intentionally precipitating solid material.
43. A non-chromatographic method as described in claim 1 wherein said step of intentionally precipitating a material within said vessel comprises the step of intentionally precipitating gel or viscous liquid.
44. A non-chromatographic method as described in claim 1 wherein said method is accomplished, at least in part, with a flow system.
45. A non-chromatographic method as described in claim 44 wherein said flow system is a continuous flow system.
46. A non-chromatographic method as described in claim 1 wherein said step of inputting an aqueous, polar precipitant solvent into said vessel through at least one vessel inlet comprises the step of inputting a liquid into said vessel through at least one vessel inlet.
47. A non-chromatographic method as described in claim 1 wherein said step of inputting an aqueous, polar, precipitant solvent into said vessel through at least one vessel inlet comprises the step of inputting gel or viscous liquid.
48. A non-chromatographic method as described in claim 1 wherein said method is an automated method.
49. A non-chromatographic method as described in claim 1 wherein said material comprises impurities in water.
50. A non-chromatographic method as described in claim 1 wherein said method is selected from the group of methods consisting of: a separation method; a processing method; a fractionating method; a production method; a distillation method; a blending method; and a measurement method.