Promiscuous HER-2/Neu CD4 T cell epitopes
The present invention relates to the discovery of novel T cell epitopes of the human HER-2/Neu protein that is promiscuous for at least 25 different HLA-DR alleles. The invention also relates to compositions that contain one of the novel epitopes or a fusion peptide of such a epitope and a heterologous polypeptide. Further disclosed herein is the use of the epitopes or their fusion peptides, and compositions containing the epitopes or their fusion peptides.
1. An isolated nucleic acid consisting of a polynucleotide sequence encoding the amino acid sequence of SEQ ID NOs: 2, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 31.
2. An isolated nucleic acid comprising a polynucleotide sequence encoding a fusion peptide consisting of the amino acid sequence of SEQ ID NOs: 2, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 31 and a heterologous polypeptide.
3. The nucleic acid of claim 1 or 2 , wherein the polynucleotide sequence encodes the amino acid sequence of SEQ ID NO:2.
4. The nucleic acid of claim 2 , wherein the heterologous polypeptide is a granulocyte-macrophage colony-stimulating factor (GM-CSF).
5. An expression cassette comprising the nucleic acid of claim 1 or 2 .
6. The expression cassette of claim 5 , which is a recombinant viral vector.
7. The expression cassette of claim 5 , which directs the expression of the nucleic acid of claim 1 or 2 .
8. A host cell comprising the expression cassette of claim 5 .
9. A host cell comprising the expression cassette of claim 6 .
10. The isolated nucleic acid of claim 1 , wherein the polynucleotide sequence encodes the amino acid sequence of SEQ ID NOs: 20, 21, 22, or 23.
11. The isolated nucleic acid of claim 1 , wherein the polynucleotide sequence encodes the amino acid sequence of SEQ ID NOs: 24, 25, or 26.
12. The isolated nucleic acid of claim 1 , wherein the polynucleotide sequence encodes the amino acid sequence of SEQ ID NOs: 27, 28, 29, or 31.