Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol

1. A non-naturally occurring microbial organism, having a 1,3-butanediol (1,3-BDO) pathway, wherein said microbial organism comprises an exogenous nucleic acid encoding each of the following a 1,3-BDO pathway enzymes or proteins expressed in a sufficient amount to produce 1,3-BDO:

1) formate dehydrogenase that catalyzes the incorporation of CO2 into formate;

2) formyltetrahydrofolate synthetase that ligates formate to tetrahydrofolate to form 10-formyltetrahydro folate;

3) methenyltetrahydrofolate cyclohydrolase that converts 10-formyltetrahydrofolate to methyltetrahydrofolate;

4) methylenetetrahydrofolate dehydrogenase that converts methyltetrahydrofolate to methenyltetrahydrofolate;

5) methylenetetrahydrofolate reductase that converts metheneyltetrahydrofolate to 5-methyltetrahydrofolate;

6) methyltetrahydrofolate:corrinoid protein methyltransferase that catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to corrinoid iron sulfur protein;

7) corrinoid iron-sulfur protein;

8) nickel-protein assembly protein;

9) ferredoxin;

10) acetyl-CoA synthase that catalyzes the condensation of the methylated corrinoid iron sulfur protein, carbon monoxide and coenzyme A, yielding acetyl-CoA;

11) carbon monoxide dehydrogenase that converts CO and water to CO2 while passing the electrons to a reduced acceptor;

12) hydrogenase that transfers electrons from H2 to an acceptor;

13) acetoacetyl-CoA thiolase that converts acetyl-CoA to acetoacetyl-CoA;

14) acetoacetyl-CoA reductase (ketone reducing) that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA;

15) 3-hydroxybutyryl-CoA reductase (aldehyde forming) that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde; and

16) 3-hydroxybutyraldehyde reductase that converts 3-hydroxybutyraldehyde to 1,3-BDO.

2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism uses a carbon feedstock selected from 1) CO, 2) CO 2 and H 2 , 3) CO, CO 2 , and H 2 , 4) synthesis gas comprising CO and H 2 , 5) synthesis gas comprising CO, CO 2 , and H 2 , and 6) one or more carbohydrates.

3. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is CO.

4. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is CO 2 and H 2 .

5. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is CO, CO 2 , and H 2 .

6. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is synthesis gas comprising CO and H 2 .

7. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is synthesis gas comprising CO, CO 2 , and H 2 .

8. The non-naturally occurring microbial organism of 2 , wherein the carbon feedstock is one or more carbohydrates.