Polymers for functional particles
View Patent ↗The present invention generally relates to polymers and macromolecules, in particular, to block polymers useful in particles such as nanoparticles. One aspect of the invention is directed to a method of developing nanoparticles with desired properties. In one set of embodiments, the method includes producing libraries of nanoparticles having highly controlled properties, which can be formed by mixing together two or more macromolecules in different ratios. One or more of the macromolecules may be a polymeric conjugate of a moiety to a biocompatible polymer. In some cases, the nanoparticle may contain a drug. The moiety, in some embodiments, may have a molecular weight greater than about 1000 Da; for example, the moiety may include a polypeptide or a polynucleotide, such as an aptamer. The moiety may also be a targeting moiety, an imaging moiety, a chelating moiety, a charged moiety, or a therapeutic moiety. Another aspect of the invention is directed to systems and methods of producing such polymeric conjugates. In some embodiments, a solution containing a polymer is contacted with a liquid, such as an immiscible liquid, to form nanoparticles containing the polymeric conjugate. Other aspects of the invention are directed to methods using such libraries, methods of using or administering such polymeric conjugates, methods of promoting the use of such polymeric conjugates, kits involving such polymeric conjugates, or the like.
1. A nanoparticulate composition comprising a particle having an average characteristic dimension of less than about 1 micrometer, the particle comprising an amphiphilic macromolecule, the macromolecule comprising a first hydrophobic portion, a second hydrophilic portion, and a third portion comprising a targeting moiety or an antigenic moiety, the first, second or both portions comprising a biocompatible polymer, wherein the particle is formed by a process which orients the third portion moiety to the surface of the nanoparticle, and wherein there is a detectable amount of the moiety in the interior of the particle.
2. The composition of claim 1 , wherein the moiety has a molecular weight of at least about 1000 Da.
3. The composition of claim 1 , wherein the moiety has a molecular weight of no more than about 1000 Da.
4. The composition of claim 1 , wherein the polymer is a block copolymer.
5. The composition of claim 1 , wherein the polymer is amphiphilic.
6. The composition of claim 1 , wherein at least a portion of the macromolecule is biodegradable.
7. The composition of claim 6 , wherein at least a portion of the macromolecule is hydrolyzable.
8. The composition of claim 1 , wherein the biocompatible polymer comprises a polymer selected from the group consisting of poly(lactide-co-glycolide), poly(lactide), poly(glycolide), poly(orthoesters), poly(caprolactones), polylysine, poly(ethylene imine), poly(acrylic acid), poly(urethanes), poly(anhydrides), poly(esters), poly(trimethylene carbonate), poly(ethyleneimine), poly(beta amino esters), and copolymers thereof.
9. The composition of claim 1 , wherein the targeting moiety comprises a molecule selected from the group consisting of a polypeptide, a nucleic acid, a fatty acid, a carbohydrate, a peptidoglycan, and a glycopeptide.
10. The composition of claim 1 , wherein the targeting moiety comprises an antibody or an antibody fragment.
11. The composition of claim 1 , wherein the targeting moiety is able to specifically bind to a molecule on the surface of a cell.
12. The composition of claim 1 , wherein the targeting moiety is able to specifically bind to a biological entity selected from the group consisting of bacterial membrane proteins, cytokines, chemokines, growth factors, insulin, erythropoietin, tumor necrosis factor, glycoproteins, adhesion molecules, fibronectins, laminin, and antigens.
13. The composition of claim 1 , wherein the nanoparticle further comprises a second macromolecule comprising the biocompatible polymer without the moiety.
14. The composition of claim 1 , wherein the nanoparticle comprises an antigen.
15. The composition of claim 1 , wherein the nanoparticle has an average characteristic dimension of less than about 150 nm.
16. The composition of claim 4 , wherein the block copolymer further comprises a third block comprising a poly(alkylene glycol).
17. The composition of claim 16 , wherein the poly(alkylene glycol) comprises poly(ethylene glycol).
18. The composition of claim 1 , wherein the nanoparticle is made by a process comprising:
providing a solution comprising the macromolecule; and
contacting the solution with a polymer nonsolvent wherein the macromolecules self-assemble to produce the particle.
19. The composition of claim 18 , wherein the solution comprising the macromolecule is an organic solution and the polymer nonsolvent is an aqueous solution.
20. The composition of claim 1 comprising a
nanoparticle comprising a first macromolecule and a second macromolecule;
wherein the first macromolecule is a block copolymer comprising a first biocompatible polymer, a poly(alkylene glycol), and a moiety selected from the group consisting of a targeting moiety and an antigen; and
wherein the second macromolecule is a block copolymer comprising a poly(alkylene glycol).