Process for manufacturing a sweetener and use thereof
Highly purified Stevioside, Rebaudioside A and a purified sweet steviol glycoside mixture were prepared from sweet glycoside extracts obtained from Stevia rebaudiana Bertoni leaves. The resulting sweeteners are suitable as non-calorie, non-cariogenic, non-bitter, non-lingering sweeteners, which may be advantageously applied in foods, beverages, and milk products.
1. A process for producing a liquid low-calorie sweetener composition, comprising the steps of:
a) drying Stevia rebaudiana leaves;
b) treating the dried Stevia rebaudiana leaves with water to extract sweet glycosides, wherein a leaves-water mixture is formed;
c) filtering the leaves-water mixture to obtain an aqueous filtrate;
d) directly treating the aqueous filtrate with calcium hydroxide or calcium oxide to raise the pH of the aqueous filtrate to about 10;
e) directly neutralizing the treated aqueous filtrate with trivalent iron chloride; and
f) filtrating the neutralized aqueous filtrate to obtain a filtrated aqueous filtrate, and evaporating the filtrated aqueous filtrate to dryness to obtain purified sweet glycosides to provide a high intensity sweetener;
g) providing sugar granules as a low intensity sweetener;
h) dissolving the high intensity sweetener and low intensity sweetener in water to obtain a solution with 45-55% total solids; and
i) evaporating the solution of high intensity sweetener and low intensity sweetener under vacuum to obtain the liquid low-calorie sweetener composition with a total solids content of about 60% to about 90%.
2. The process claim 1 , wherein a dry weight ratio of the low intensity sweetener to high intensity sweetener is in the range of about 50:1 to about 300:1 (w/w).
3. The process of claim 1 , wherein the purified sweet glycosides obtained in step (f) are dissolved in methanol at temperatures ranging from about 20-50° C., in an amount of 1:2-1:7 (w/v) to precipitate Stevioside from solution, filtering the solution to recover Stevioside precipitate, suspending the precipitate in an alcohol-water solution to further purify the Stevioside, and recovering the higher purity of Stevioside with a purity of at least 98% to provide the high intensity sweetener.
4. The process of claim 3 , further comprising the steps of combining remaining alcohol solutions, removing the alcohol, ultrafiltering to obtain a filtrate, recovering Rebaudioside A, mixing the Rebaudioside A with dilute alcohol, and filtering and drying to result in Rebaudioside A with a purity of at least 98% to provide the high intensity sweetener.