MicroRNA modulators and method for identifying and using the same
View Patent ↗The present invention is a method for identifying agents which modulate microRNA activity. The invention involves contacting a cell harboring a microRNA and a microRNA binding sequence, which is operably linked to a nucleic acid molecule encoding a reporter protein, with a test agent increases or decreases the expression of the reporter protein thereby identifying a microRNA modulator. Antagonists identified by this screening assay are provided, as are methods for using the same to inhibit microRNA activity and prevent or treat disease.
1. An miR-21 antagonist having the structure of compound 2:
2. A pharmaceutical composition comprising the miR-21 antagonist having the structure of compound 2:
in admixture with a pharmaceutically acceptable carrier.