Determination of particle properties
The present invention relates to a method and system for determining particle properties. Such properties may for example be charge, size, drift, etc. The method comprises determining ( 110 ) an electric mobility distribution based on detection of individual particles. The latter may be performed for a single particle over time, for a plurality of particles at the same time or in a combination thereof. The method also comprises deriving a particle property based on a periodicity in the electric mobility distribution.
1. A method for determining particle properties of particles in a liquid, the method comprising the steps:
determining an electric mobility distribution, said distribution being a distribution of the electric mobility of individual particles, based on determination of a plurality of values for the electric mobility of an individual particle, the determining being based on detection of individual particles, and
deriving a particle property based on a periodicity in the electric mobility distribution, the periodicity being characteristic for an electrical characteristic μ e , said characteristic being the elementary mobility of a particle with charge e.
2. The method according to claim 1 , wherein the periodicity is caused by jumps over a value μ e being the elementary mobility of a particle corresponding with the difference in mobility when a particle charge reduces or increase with a single elementary charge.
3. The method according to claim 1 , wherein the electric mobility is determined in an electrical field having an electric field amplitude of 10 6 V/m or higher.
4. The method according to claim 1 , wherein determining a plurality of values for the electric mobility of an individual particle comprises determining a plurality of values for one individual particle over time.
5. The method according to claim 1 , wherein determining a plurality of values for the electric mobility of an individual particle comprises determining for a plurality of particles a value of an electric mobility for each particle individually.
6. The method according to claim 1 , wherein determining a plurality of values for the electric mobility of an individual particle comprise performing an electrophoretic measurement on at least one individual particle.
7. The method according to claim 1 , wherein determining a plurality of values of the electric mobility comprises applying an electric field and detecting the motion of at least one particle as function of the electric field so as to determine the electric mobility.
8. The method according to claim 7 , wherein detecting the motion of the particle is performed by optically monitoring the motion of at least one particle.
9. The method according to claim 1 , wherein the particle property is any of a charge or a size.
10. The method according to claim 1 , comprising:
deriving a property of the liquid in which the particles are dispensed, based on the determined particle property.
11. The method according to claim 1 for use in a biosensing method for detecting a biological, chemical or bio-chemical event.
12. A system for determining particle properties of particles in a liquid, the system comprising a means adapted for determining an electric mobility distribution, said distribution being a distribution of the electric mobility of individual particles, based on determination of a plurality of values for the electric mobility of an individual particle, the means adapted for determining being based on detection of individual particles and a means adapted for deriving a particle property based on a periodicity in the electric mobility distribution, the periodicity being characteristic for an electrical characteristic μ e , said characteristic being the elementary mobility of a particle with charge e.
13. The system according to claim 12 , wherein the means adapted for determining an electric mobility distribution comprises an electric field generating means and a detection means adapted for detecting movement of at least one particle.
14. The system according to claim 13 , wherein the system furthermore comprises a measurement channel for introducing the particles dispensed in a liquid, the portion over which an electric field is placed having characteristic dimensions smaller than 250 μm.
15. The system according to claim 14 , the system comprising a biosensor for sensing a biological, chemical or biochemical event.
16. A controller adapted for controlling operation of a system according to a method for determining particle properties, the method comprising:
determining an electric mobility distribution, said distribution being a distribution of the electric mobility of individual particles, based on determination of a plurality of values for the electric mobility of an individual particle, the determining being based on detection of individual particles, and
deriving a particle property based on a periodicity in the electric mobility distribution, the periodicity being characteristic for an electrical characteristic μ e , said characteristic being the elementary mobility of a particle with charge e.
17. The controller according to claim 16 , the controller comprising a computer program product adapted to perform the method for determining particle properties, when executed on a computing device.