Therapeutic composition produced using punica granatum and hydrogen peroxide
Methods of and compositions for producing and using plant-based materials are provided. The methods include using biopolymers or their synthetic equivalents combined with a stable source of reactive oxygen species that when applied to or combined with a separate source of oxido-reducing enzyme or catalyst will cause the formation of an activated biopolymer with increased protein binding affinity and microbial control activities.
1. An anti-bacterial composition consisting of a product prepared by:
a) combining pomegranate rind with water and heating at 80° C. to 150° C. to substantially denature enzymes endogenous to said pomegranate rind and
b) adding from 1% to less than 10% of hydrogen peroxide to the product obtained from part (a).
2. The anti-bacterial composition of claim 1 , wherein the composition endogenously contains a component selected from the group consisting of a tannin, a lignin, a flavonoid, a hydroxycoumarin and an alkaloid.
3. The anti-bacterial composition of claim 1 , wherein said composition is in a dose ranging from 20 μg to 250 μg.
4. The anti-bacterial composition of claim 1 , wherein the heating in part (a) is carried out from 80° C. to 110° C.
5. The anti-bacterial composition of claim 1 , wherein 1% to 2% of hydrogen peroxide is added in part (b).
6. A kit comprising a dry form of the anti-bacterial composition of any one of claims 1 - 5 .
7. A method for treating a bacterial infection, comprising administering to a subject in need thereof, an effective amount of the anti-bacterial composition of any one of claims 1 - 5 .
8. A method of treating bacteria-infected water, comprising administering to said water an effective amount of the anti-bacterial composition of any one of claims 1 - 5 .
9. A method of treating or preventing a bacterial infection in a wounded tissue, comprising administering to said tissue, an effective amount of the anti-bacterial composition of any one of claims 1 - 5 .
10. A method for treating a bacterial-infected tissue, comprising administering to said tissue, an effective amount of the composition of any one of claims 1 - 5 .
11. A method for producing an anti-bacterial composition, wherein the method comprises;
a) combining pomegranate rind with water and heating at 80° C. to 150 ° C. to substantially denature enzymes endogenous to said pomegranate rind and
b) adding from 16% to less than 10% of hydrogen peroxide to the product obtained from part (a).
12. The method of claim 11 , wherein said heating in part (a) is carried out from 80° C. to 110° C.
13. The method of claim 11 , wherein 1% to 2% of hydrogen peroxide is added in part (b).
14. The method of claim 11 , wherein the method further comprises creating 20 μg to 250 μg dosage forms of said anti-bacterial composition.
15. An anti-bacterial composition consisting of a product prepared by:
a) combining pomegranate rind with water and heating at 80° C. to 150° C. to substantially denature enzymes endogenous to said pomegranate rind,
b) adding from 1% to less than 10% of hydrogen peroxide to the product obtained from part (a) and
c) filtering the product obtained from part (b).