Microparticle analysis device and microparticle analysis method
Disclosed herein is a microparticle analysis device including: a light source configured to irradiate a microparticle with light; an acousto-optic modulator configured to diffract fluorescence generated from the microparticle due to the light irradiation; a slit configured to allow transmission of only diffracted light in a diffraction center wavelength region among diffracted light beams from the acousto-optic modulator; and a detector configured to detect the diffracted light in the diffraction center wavelength region transmitted through the slit.
1. A microparticle analysis device comprising:
a light source configured to irradiate a microparticle with light;
an acousto-optic modulator configured to diffract fluorescence generated from the microparticle due to the light irradiation;
a slit configured to allow transmission of only diffracted light in a diffraction center wavelength region among diffracted light beams from the acousto-optic modulator;
a detector configured to detect the diffracted light in the diffraction center wavelength region transmitted through the slit;
a controller configured to apply an acoustic wave to the acousto-optic modulator and discontinuously switch frequency of the acoustic wave; and
a detector configured to detect scattered light generated from the microparticle due to the light irradiation, wherein
the controller discontinuously switches the frequency of the acoustic wave applied to the acousto-optic modulator based on input of a detection signal from the detector to detect the scattered light.
2. A microparticle analysis method comprising:
diffracting fluorescence generated from a microparticle due to light irradiation by an acousto-optic modulator that provides a diffraction center wavelength region made to correspond with a detected-fluorescence wavelength region;
making diffracted light from the acousto-optic modulator be transmitted through a slit that allows transmission of only diffracted light in the diffraction center wavelength region;
detecting the diffracted light in the diffraction center wavelength region transmitted through the slit; and
discontinuously switching frequency of an acoustic wave applied to the acousto-optic modulator, and
wherein the frequency of the acoustic wave applied to the acousto-optic modulator is discontinuously switched during detection of scattered light generated from the microparticle due to the light irradiation.