Oligonucleotide derivative, labeling agent and use for labeling agent
A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group, X 1 represents O, S or Se, and X 2 represents SH (or S − ), Se − , a C 1-4 alkyl or a morpholino group).
1. A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below:
(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X 1 represents O, S or Se, and X 2 represents SH (or S − ), Se − , or a C 1-4 alkyl group or morpholino group).
2. The labeling agent according to claim 1 , wherein said Y is a condensed polycyclic hydrocarbon group.
3. The labeling agent according to claim 2 , wherein said Y is selected from a group consisting of naphthalene, phenathrene, pyrene and perylene.
4. The labeling agent according to claim 1 , comprising a plurality of said nucleotide derivative units in a continuous series.
5. The labeling agent according to claim 1 , comprising a plurality of said nucleotide derivative units, with linker units in between.
6. The labeling agent according to claim 1 , wherein said multiple nucleotide units are two nucleotide derivative units in which said Y is the same or different, and are provided in a continuous series.
7. A set of labeling agents comprising two or more labeling agents each provided with a plurality of nucleotide derivative units represented by Formula (1) below:
(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X 1 represents O, S or Se, and X 2 represents SH (or S − ), Se − , or a C 1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the labeling agents.
8. The set of labeling agents according to claim 7 , further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1) above.
9. An oligonucleotide probe comprising a plurality of nucleotide derivative units represented by Formula (1) below:
(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X 1 represents O, S or Se, and X 2 represents SH (or S − ), Se − , or a C 1-4 alkyl group or morpholino group).
10. The oligonucleotide probe according to claim 9 , comprising said plurality of nucleotide derivative units at the 3′ terminal end or 5′ terminal end.
11. An oligonucleotide probe set comprising two or more oligonucleotide probes each provided with a plurality of nucleotide derivative units represented by Formula (1) below:
(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X 1 represents O, S or Se, and X 2 represents SH (or S − ), Se − or a C 1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the probes.
12. The oligonucleotide probe set according to claim 11 , further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1).