Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
Disclosed are major allergenic proteins in cashew nut, which are legumin-like proteins and 2S albumins. Also disclosed is a polypeptide allergen in the 7S superfamily, which includes vicilin-like and sucrose binding proteins. Several linear epitopes of the cashew nut are identified and characterized. The invention further discloses the sequence of cDNA encoding the allergenic polypeptide, the allergen being designated Ana o 1, and also describes the characterization of the expressed recombinant polypeptide and associated methods employing the polypeptide.
1. A diagnostic test for detecting anti-cashew IgE in a patient to thereby indicate an allergy to cashews, said test comprising:
reacting patient's serum with a composition comprising a purified polypeptide sequence consisting of SEQ ID NO: 14;
separating the polypeptide from unreacted patient serum;
reacting the polypeptide with a labeled human IgE-reactive agent after separating from unreacted patient serum;
separating the polypeptide from unreacted labeled human IgE-reactive agent; and
directly or indirectly detecting the labeled human IgE-reactive agent bound to the polypeptide after separating from unreacted agent to thereby indicate presence in the patient's serum of anti-cashew IgE.