Preparation for treating heart disease used in cell therapy
An object of the present invention is to establish a cell transplantation method which can markedly improve the survival of grafted pluripotent stem cells and the efficiency of cardiomyocyte regeneration in cell therapy using pluripotent stem cells derived from heart tissue and can treat heart disease further effectively. Specifically, according to the present invention, enhancement in the survival of grafted pluripotent stem cells and significant improvement in the efficiency of cardiomyocyte regeneration are achieved by using, in combination, pluripotent stem cells derived from heart tissue and a hydrogel containing a basic fibroblast growth factor (bFGF) in cell therapy for heart disease.
1. A preparation for treating heart disease used in cell therapy, comprising: a hydrogel in a sheet form containing a basic fibroblast growth factor; and
pluripotent stem cells derived from human heart tissue, wherein the preparation is formulated such that the basic fibroblast growth factor is administered in an amount of at least 1 to 100 μg with respect to the amount of the pluripotent stem cells of 1×10 6 cells.
2. The preparation according to claim 1 , wherein the preparation is formulated as a kit comprising the hydrogel and the pluripotent stem cells.
3. The preparation according to claim 1 , wherein the hydrogel is at least one hydrogel selected from the group consisting of gelatin, collagen, starch, pectin, hyaluronic acid, chitin, chitosan, and alginic acid, and derivatives of these materials.
4. The preparation according to claim 3 , wherein the hydrogel is a collagen or gelatin hydrogel.
5. The preparation according to claim 1 , wherein the preparation is formulated such that the dose of the hydrogel containing a basic fibroblast growth factor is 1 to 100 μg per kg body weight of a patient in terms of the amount of the basic fibroblast growth factor.
6. The preparation according to claim 1 , further comprising a non-biodegradable polymer support.
7. The preparation according to claim 6 , wherein the hydrogel is immobilized on the non-biodegradable polymer support when the preparation is administered.
8. The preparation according to claim 7 , wherein the non-biodegradable polymer support is polytetrafluoroethylene.
9. The preparation according to claim 1 , wherein the preparation is formulated such that the pluripotent stem cells are administered in an amount of 1×10 6 cells or less per kg body weight of a patient.
10. The preparation according to claim 9 , wherein the preparation is formulated such that the pluripotent stem cells are administered in an amount of 1×10 5 to 10×10 5 cells per kg body weight of a patient.
11. The preparation according to any of claim 1 , wherein the pluripotent stem cells are CD90-positive, CD29-positive, CD73-positive, stro-1-positive, and CD105-positive.
12. The preparation according to claim 1 , wherein the pluripotent stem cells are derived from heart tissue of a patient.
13. The preparation according to claim 1 , wherein the pluripotent stem cells are derived from heart tissue other than that of a patient.
14. The preparation according to claim 1 , wherein the pluripotent stem cells are an established cell line.
15. A method for treating heart disease, comprising:
administering to a patient the preparation according to claim 1 .
16. The method according to claim 15 , wherein the preparation hydrogel is immobilized on a non-biodegradable polymer support.
17. The method according to claim 15 , wherein the preparation is formulated such that the pluripotent stem cells are administered in an amount of 1×10 5 to 10×10 5 cells per kg body weight of a patient.
18. The method according to claim 15 , wherein the preparation is formulated such that the pluripotent stem cells are administered in an amount of 1×10 6 cells or less per kg body weight of a patient.
19. The preparation according to claim 11 , wherein the pluripotent stem cells are c-kit-negative.