Granted Patent B2

US 8,415,165 · App. 11/954,051 · Granted Apr 9, 2013

Inventors: Ming-Hwa Liang (Stony Brook, NY); Stephane Shu Kin So (Stony Brook, NY); James Hayward (Stony Brook, NY)

Assignee: APDN (B.V.I.), Inc.

Abstract

Claims (150)

1. A sports garment authentication method comprising:

providing the sports garment or a piece of the sports garment, wherein the sports garment or the piece of the sports garment comprises a nucleic acid material having an artificial sequence obtained by digestion and ligation of extracted DNA and having a particular sequence of nucleic acid bases associated with said sports garment, the nucleic acid material being linked by a linking group comprising an alkylene moiety, to an upconverting phosphor particle;

obtaining a sample of said particular nucleic acid material;

detecting the particular sequence of nucleic acid bases of the nucleic acid material by performing a polymerase chain reaction (PCR) on the nucleic acid material; and

verifying said sports garment or piece of said sports garment is genuine by detecting said particular sequence of nucleic acid bases of the nucleic acid material obtained from said sports garment or said piece of sports garment.

2. The method of claim 1 wherein the nucleic acid material is applied to the surface of the piece of the sports garment.

3. The method of claim 1 wherein the nucleic acid material is applied to a specific area on the surface of the piece of the sports garment.

4. The method of claim 3 wherein said marking comprises an indicia or a logo.

5. The method of claim 1 wherein the piece of the sports garment is selected from the group consisting of a sport bib, a racing bib, and a removable sports identification patch.

6. The method of claim 1 wherein the nucleic acid material is deoxyribonucleic acid (DNA).

7. The method of claim 1 wherein the nucleic acid material is ribonucleic acid (RNA).

8. A method for authenticating sports garment comprising the steps of:

providing a sports garment having an associated optical reporter marker, the optical reporter marker having at least one light emitting upconverting phosphor particle linked by a linking group comprising an alkylene moiety, to at least one nucleic acid material having an artificial sequence obtained by digestion and ligation of extracted DNA, the nucleic acid material having an identifiable portion,

detecting the optical reporter marker associated with the garment with a light source,

obtaining a sample of the optical reporter marker from the garment of interest for analysis; and

analyzing the collected sample to detect the presence of the identifiable portion of the nucleic acid material linked to the upconverting phosphor particle.

9. The method of claim 8 , wherein the optical reporter marker has the composition of the formula I:

(cOpR)-[L-(NA)] m   I

wherein:

m is an integer greater than 1;

(cOpR) is a coated optical reporter particle;

(NA) is a nucleic acid oligomer of the nucleic acid material of detectable sequence; and

L is the linking group covalently bound to the coated optical reporter particle and to the nucleic acid material.

10. The method of claim 9 , wherein (NA) is a single stranded DNA molecule having a length of from about 40 bases to about 1000 bases, or a double stranded DNA molecule having a length of from about 40 base pairs to about 1000 base pairs.

11. The method of claim 9 , wherein L comprises a C 2-8 alkylene moiety having a first end covalently bound to the coated optical reporter particle and a second end covalently bound to the nucleic acid oligomer.

12. The method of claim 9 , wherein the (UCP) upconverting phosphor particle is of the formula:

Y x Yb y Er z O 2 S; or

Na(Y x Yb y Er z )F 4 ;

wherein:

x is from about 0.6 to about 0.95;

y is from about 0.05 to about 0.35; and

z is from about 0.1 to about 0.001.

13. The method of claim 9 , wherein L is of the formula:

-A-R 1 —B—

wherein:

R 1 is C 2-8 alkylene;

-A- is a group covalently bonded to the surface of the coated optical reporter; and

—B— is a group covalently bonded to the 3′ or 5′ end of the nucleic acid oligomer.

14. A method for authenticating a sports garment comprising the steps of;

providing a sports garment having an associated optical reporter marker, the optical reporter marker having at least one light emitting upconverting phosphor particle linked by a linking group comprising an alkylene moiety, to a nucleic acid taggant, the nucleic acid taggant having a plurality of artificial polymorphic double stranded (ds) DNA fragments each having an artificial sequence obtained by restriction digestion and ligation of extracted DNA, said artificial polymorphic dsDNA fragments having an identifiable portion,

detecting the optical reporter marker associated with the sports garment with a light source,

obtaining a sample of the optical reporter marker from the sports garment for analysis,

analyzing the sample to detect the presence of the identifiable portion of the artificial polymorphic (ds)DNA fragments linked to the upconverting phosphor particle by performing a polymerase chain reaction (PCR) on the collected sample comprising said artificial polymorphic (ds)DNA fragments using specific primers, thereby producing specific length amplicons; and

verifying that the sports garment is genuine by detecting the specific lengths of said specific length amplicons amplified from said artificial polymorphic dsDNA fragments in said sports garment.

15. The method of claim 14 , wherein the optical reporter marker linked to the plurality of artificial polymorphic double stranded (ds)DNA fragments has the composition of the formula I:

(cOpR)-[L-(NA)] m   I

wherein:

m is an integer greater than 1;

(cOpR) is a coated optical reporter particle, said COpR having an upconverting phosphor (UCP) material;

(NA) is a nucleic acid oligomer of detectable sequence, wherein (NA) is a single stranded DNA molecule having a length of between about 40 bases and about 1000 bases, or a double stranded DNA molecule having a length of between about 40 base pairs and about 1000 base pairs; and

L is a linking group covalently bound to the coated optical reporter particle and to the nucleic acid oligomer, wherein L has an alkylene moiety.

16. The method of claim 15 , wherein (NA) is a single stranded DNA molecule having a length of between about 100 bases and about 800 bases, or a double stranded DNA molecule having a length of between about 150 base pairs and about 500 base pairs.

17. The method of claim 15 , wherein the (UCP) upconverting phosphor particle is of the formula:

Y x Yb y Er z O 2 S; or

Na(Y x Yb y Er z )F 4 ;

wherein:

x is from about 0.6 to about 0.95;

y is from about 0.05 to about 0.35; and

z is from about 0.1 to about 0.001.

18. The method of claim 15 , wherein L is of the formula:

-A-R 1 —B—

wherein:

R 1 is C 2-8 alkylene;

-A- is a group covalently bonded to the surface of the coated optical reporter; and

—B— is a group covalently bonded to the 3′ or 5′ end of the nucleic acid oligomer.

19. The method of claim 18 , wherein -A- is —O—.

20. The method of claim 18 , wherein —R 1 — is —(CH 2 ) n — and wherein n is from 2 to 8.

21. The method of claim 18 , wherein —B— is:

—S—;

—O—;

—NR a —;

—S—(CH 2 ) p —;

—O—(CH 2 ) p —;

—NR a —(CH 2 ) p —;

—S—(CH 2 ) q —C(O)—NR a —(CH 2 ) p —;

—O—(CH 2 ) q —C(O)—NR a —(CH 2 ) p —;

—NR a —(CH 2 ) q —C(O)—NR a —(CH 2 ) p —;

—S—C(O)—(CH 2 ) r —C(O)—NR a —(CH 2 ) p —;

—O—C(O)—(CH 2 ) r —C(O)—NR a —(CH 2 ) p —; or

—NR a —C(O)—(CH 2 ) r —C(O)—NR a —(CH 2 ) p —;

wherein:

p is from 2 to 8;

q is from 1 to 8;

r is from 2 to 8; and

each R a is independently hydrogen or C 1-6 alkyl.

22. The method of claim 18 , wherein —B— is:

—S—(CH 2 ) q —C(O)—NR a —(CH 2 ) p or

—NR a —C(O)—(CH 2 ) r —C(O)—NR a —(CH 2 ) p —.

23. The method of claim 22 , wherein:

p is from 2 to 6;

q is from 1 to 3; and

r is 2 or 3.

24. The method of claim 18 , wherein —B— is:

—S—CH 2 —C(O)—NH—(CH 2 ) 6 —; or

—NH—C(O)—(CH 2 ) 3 —C(O)—NH—(CH 2 ) 6 —.

25. The method of claim 18 , wherein the cOpR is coated with silica.

26. The method of claim 25 , wherein cOpR is a coated optical reporter particle having an upconverting phosphor (UCP) material comprising Yttrium oxysulfide.

27. The method of claim 14 , wherein the optical reporter marker has the composition of the formula II:

(UCP)-[A-R 1 —X—R 2 —C(O)—NR a —R 3 -(DNA)] m   II

wherein:

m is an integer greater than 1;

UCP is an upconverting phosphor particle;

DNA is a double stranded deoxyribonucleic acid fragment;

-A- is a group capable of covalently bonding to the surface of the Upconverting phosphor particle;

R 1 is C 2-8 alkylene,

R 2 is C 1-8 alkylene or —C(O)—C 1-8 alkylene-;

—X— is —O—, —S— or —NR a —;

R 3 is C 2-8 alkylene; and

R a is hydrogen or C 1-6 alkyl.

28. The method of claim 27 , wherein the optical reporter marker has the composition of the formula IV:

(UCP)-[O—(CH 2 ) s —S—(CH 2 ) t —C(O)—NH—(CH 2 ) v -(DNA)] m   IV

wherein:

s is from 2 to 6;

v is from 2 to 6;

t is from 1 to 3.

29. The method of claim 27 , wherein the optical reporter marker has the composition of the formula V:

(UCP)-[O—(CH 2 ) s —NH—C(O)—(CH 2 ) u —C(O)—NH—(CH 2 ) v -(DNA)] m   V

wherein:

s is from 2 to 6;

v is from 2 to 6;

u is 2 or 3.

30. The method of claim 27 , wherein the optical reporter marker has the composition of the formula VI:

(UCP)-[O—(CH 2 ) 3 —S—CH 2 —C(O)—NH—(CH 2 ) 6 -(DNA)] m   VI.

31. The method of claim 27 , wherein the optical reporter marker has the composition of the formula VII:

(UCP)-[O—(CH 2 ) 3 —NH—C(O)—(CH 2 ) 3 —C(O)—NH—(CH 2 ) 6 -(DNA)] m   VII.

32. The method of claim 15 , wherein the cOpR comprises a visually detectable light emitting material selected from the group consisting of a fluorescent dye, a upconverting phosphor, a rare earth doped-ceramic powder, and a quantum dot.

33. The method of claim 32 , wherein said light emitting material is excitable by UV or infrared light.

34. The method of claim 15 , wherein the cOpR comprises at least one electromagnetic radiation-emitting material.

35. The composition of claim 34 , where the electromagnetic radiation-emitting material is detectable using a device which selected from the group consisting of an infrared radiation source, a magnetic field source, a quantum dot and an electromagnetic pulse source.

36. The method of claim 14 , wherein the nucleic acid taggant is ds DNA.

37. The method of claim 14 , wherein the sports garment is selected from the group of a manufactured sporting good, a sport bib, a hat, sport logo on fabric, sport shorts, and a wash tag on a sporting good.

38. The method of claim 14 , wherein said authenticating further comprises associating the optical reporter marker with the sports garment in a database management system.

39. A method for authenticating a sports garment, comprising:

providing a optical reporter marker linked by a linking group comprising an alkylene moiety, to at least one nucleic acid taggant;

applying the marker compound to at least one sports garment, which sports garment enters at least one supply chain;

locating and collecting a sample of the optical reporter marker from said sports garment after said sports garment has entered said supply chain; and

identifying said nucleic acid taggant from said sports garment.

40. A kit for authenticating sports garment comprising:

a container comprising an optical reporter marker linked by a linking group comprising an alkylene moiety, to at least one nucleic acid taggant; and

an applicator for applying a sample of the optical reporter to the sports garment.

41. A method for authenticating a sports garment comprising the steps of;

providing a sports garment having an associated optical reporter marker, the optical reporter marker having at least one light emitting upconverting phosphor particle covalently linked by a linking group comprising an alkylene moiety, to a nucleic acid taggant, the nucleic acid taggant having a plurality of polymorphic DNA fragments each having an artificial sequence obtained by restriction digestion and ligation of extracted DNA, at least one of said polymorphic DNA fragments having an identifiable portion,

detecting the optical reporter marker associated with the sports garment with a light source,

obtaining a sample of the optical reporter marker from the sports garment for analysis,

analyzing the collected sample to detect the presence of the identifiable portion of the at least one polymorphic DNA fragment having an identifiable portion and covalently linked to the upconverting phosphor particle by performing a polymerase chain reaction (PCR) with specific primers to produce specific amplicons and hybridizing the specific amplicons with a probe specific to the identifiable portion; and

thereby verifying that the sports garment is genuine by detecting said identifiable portion in the specific amplicons.

42. The method of claim 14 , wherein said artificial polymorphic dsDNA fragments having an identifiable portion are non-heritable DNA fragments.

43. The method of claim 14 , wherein the detecting the specific lengths of said specific length amplicons amplified from said artificial polymorphic dsDNA fragments in said sports garment comprises a capillary electrophoresis step.

44. The method of claim 41 , wherein said at least one polymorphic DNA fragment having an identifiable portion is a non-heritable DNA fragment.

45. The method of claim 41 , wherein the probe specific to the identifiable portion of the polymorphic DNA fragment is a fluorescent probe.

Assignments (8)

Continuity (8)